دورية أكاديمية
Molecular diagnosis of Chagas disease: a systematic review and meta-analysis
| العنوان: | Molecular diagnosis of Chagas disease: a systematic review and meta-analysis |
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| المؤلفون: | Guillermo Pascual-Vázquez, Montserrat Alonso-Sardón, Beatriz Rodríguez-Alonso, Javier Pardo-Lledías, Angela Romero Alegría, Pedro Fernández-Soto, Juan Luis Muñoz Bellido, Antonio Muro, Moncef Belhassen-García |
| المصدر: | Infectious Diseases of Poverty, Vol 12, Iss 1, Pp 1-18 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Chagas disease, Trypanosoma cruzi, Molecular diagnosis, Polymerase chain reaction, Loop-mediated isothermal amplification, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| الوصف: | Abstract Background The complexity of the Chagas disease and its phases is impossible to have a unique test for both phases and a lot of different epidemiological scenarios. Currently, serology is the reference standard technique; occasionally, results are inconclusive, and a different diagnostic technique is needed. Some guidelines recommend molecular testing. A systematic review and meta-analysis of available molecular tools/techniques for the diagnosis of Chagas disease was performed to measure their heterogeneity and efficacy in detecting Trypanosoma cruzi infection in blood samples. Methods A systematic review was conducted up to July 27, 2022, including studies published in international databases. Inclusion and exclusion criteria were defined to select eligible studies. Data were extracted and presented according to PRISMA 2020 guidelines. Study quality was assessed using Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2). A random-effects model was used to calculate pooled sensitivity, specificity, and diagnostic odds ratio (DOR). Forest plots and a summary of the receiving operating characteristics (SROC) curves displayed the outcomes. Heterogeneity was determined by I 2 and Tau2 statistics and P values. Funnel plots and Deek's test were used to assess publication bias. A quantitative meta-analysis of the different outcomes in the two different clinical phases was performed. Results We identified 858 records and selected 32 papers. Studies pertained to endemic countries and nonendemic areas with adult and paediatric populations. The sample sizes ranged from 17 to 708 patients. There were no concerns regarding the risk of bias and applicability of all included studies. A positive and nonsignificant correlation coefficient (S = 0.020; P = 0.992) was obtained in the set of studies that evaluated diagnostic tests in the acute phase population (ACD). A positive and significant correlation coefficient (S = 0.597; P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2049-9957 |
| Relation: | https://doaj.org/toc/2049-9957 |
| DOI: | 10.1186/s40249-023-01143-7 |
| URL الوصول: | https://doaj.org/article/3fcc33c600db410b858349fb0ee10f93 |
| رقم الأكسشن: | edsdoj.3fcc33c600db410b858349fb0ee10f93 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20499957 |
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| DOI: | 10.1186/s40249-023-01143-7 |