دورية أكاديمية
Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production
| العنوان: | Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production |
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| المؤلفون: | Eleonora Carota, Maurizio Petruccioli, Alessandro D'Annibale, Silvia Crognale |
| المصدر: | Heliyon, Vol 6, Iss 9, Pp e04801- (2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Biotechnology, Microbiology, Waste treatment, Green engineering, Sustainable development, Microbial biotechnology, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | The aqueous extraction of orange peel waste (OPW), the byproduct of the juice extraction process generated annually in massive amounts (21 Mton), yields a carbohydrate-rich liquid fraction, termed orange peel extract (OPE). Several studies highlight that the combination of glycerol, a biodiesel byproduct, with carbohydrate mixtures might boost microbial lipid production. This study performed first a shaken flask screening of 15 oleaginous yeast strains based on their growth and lipid-producing abilities on OPE- and glycerol-based media. This screening enabled the selection of R. toruloides NRRL 1091 for the assessment of the process transfer in a stirred tank reactor (STR). This assessment relied, in particular, on either single- and double-stage feeding fed-batch (SSF-FB and DSF-FB, respectively) processes where OPE served as the primary medium and nitrogen-containing glycerol-OPE mixtures as the feeding one. The continuous supply mode at low dilution rates (0.02 and 0.01 h−1 for SSF-FB and DSF-FB, respectively) starting from the end of the exponential growth of the initial batch phase enabled the temporal extension of biomass and lipid production. The SSF-FB and DSF-FB processes attained high biomass and lipid volumetric productions (LVP) and ensured significant lipid accumulation on a dry cell basis (YL/X). The SSF-FB process led to LVP of 20.6 g L−1 after 104 h with volumetric productivity (rL) of 0.20 g L−1 h−1 and YL/X of 0.80; the DSF-FB process yielded LVP, rL and YL/X values equal to 15.92 g L−1, 0.11 g L−1 h−1 and 0.65, respectively. The fatty acid profiles of lipids from both fed-batch processes were not significantly different and resembled that of Jatropha oil, a vastly used feedstock for biodiesel production. These results suggest that OPE constitutes an excellent basis for the fed-batch production of R. toruloides lipids, and this process might afford a further option in OPW-based biorefinery. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844020316443; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2020.e04801 |
| URL الوصول: | https://doaj.org/article/3fcf14954db44b969ae907dd042cf4cf |
| رقم الأكسشن: | edsdoj.3fcf14954db44b969ae907dd042cf4cf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2020.e04801 |