دورية أكاديمية
Nanotube patterning reduces macrophage inflammatory response via nuclear mechanotransduction
| العنوان: | Nanotube patterning reduces macrophage inflammatory response via nuclear mechanotransduction |
|---|---|
| المؤلفون: | Yiru Fu, Zheng Jing, Tao Chen, Xinxin Xu, Xu Wang, Mingxing Ren, Yanqiu Wu, Tianli Wu, Yuzhou Li, He Zhang, Ping Ji, Sheng Yang |
| المصدر: | Journal of Nanobiotechnology, Vol 21, Iss 1, Pp 1-16 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Titania nanotube patterning, Anodization, Mechanotransduction, Macrophage, Inflammatory response, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Abstract The inflammatory immune environment surrounding titanium bone implants determines the formation of osseointegration, and nanopatterning on implant surfaces modulates the immune microenvironment in the implant region. Among many related mechanisms, the mechanism by which nanopatterning controls macrophage inflammatory response still needs to be elucidated. In this paper, we found that inhibition of the nuclear envelope protein lamin A/C by titania nanotubes (TNTs) reduced the macrophage inflammatory response. Knockdown of lamin A/C reduced macrophage inflammatory marker expression, while overexpression of lamin A/C significantly elevated inflammatory marker expression. We further found that suppression of lamin A/C by TNTs limited actin polymerization, thereby reducing the nuclear translocation of the actin-dependent transcriptional cofactor MRTF-A, which subsequently reduced the inflammatory response. In addition, emerin, which is a key link between lamin A/C and actin, was delocalized from the nucleus in response to mechanical stimulation by TNTs, resulting in reduced actin organization. Under inflammatory conditions, TNTs exerted favourable osteoimmunomodulatory effects on the osteogenic differentiation of mouse bone marrow-derived stem cells (mBMSCs) in vitro and osseointegration in vivo. This study shows and confirms for the first time that lamin A/C-mediated nuclear mechanotransduction controls macrophage inflammatory response, and this study provides a theoretical basis for the future design of immunomodulatory nanomorphologies on the surface of metallic bone implants. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-3155 |
| Relation: | https://doaj.org/toc/1477-3155 |
| DOI: | 10.1186/s12951-023-01912-4 |
| URL الوصول: | https://doaj.org/article/c4025fadc51b4f839a16b296624da7c3 |
| رقم الأكسشن: | edsdoj.4025fadc51b4f839a16b296624da7c3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14773155 |
|---|---|
| DOI: | 10.1186/s12951-023-01912-4 |