دورية أكاديمية
Jamestown Canyon virus in Massachusetts: clinical case series and vector screening
| العنوان: | Jamestown Canyon virus in Massachusetts: clinical case series and vector screening |
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| المؤلفون: | Cormac M. Kinsella, Molly L. Paras, Sandra Smole, Samar Mehta, Vijay Ganesh, Lin H. Chen, Daniel P. McQuillen, Ruta Shah, Justin Chan, Matthew Osborne, Scott Hennigan, Frederic Halpern-Smith, Catherine M. Brown, Pardis Sabeti, Anne Piantadosi |
| المصدر: | Emerging Microbes and Infections, Vol 9, Iss 1, Pp 903-912 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Jamestown Canyon virus, arbovirus, encephalitis, meningitis, diagnostics, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | ABSTRACTJamestown Canyon virus (JCV) is a neuroinvasive arbovirus that is found throughout North America and increasingly recognized as a public health concern. From 2004 to 2012, an average of 1.7 confirmed cases were reported annually in the United States, whereas from 2013 to 2018 this figure increased over seventeen-fold to 29.2 cases per year. The rising number of reported human infections highlights the need for better understanding of the clinical manifestations and epidemiology of JCV. Here, we describe nine patients diagnosed with neuroinvasive JCV infection in Massachusetts from 2013, the year of the first reported case in the state, to 2017. Because current diagnostic testing relies on serology, which is complicated by cross-reactivity with related orthobunyaviruses and can be negative in immunosuppressed patients, we developed and evaluated an RT-qPCR assay for detection of JCV RNA. We tested this on the available archived serum from two patients, but did not detect viral RNA. JCV is transmitted by multiple mosquito species and its primary vector in Massachusetts is unknown, so we additionally applied the RT-qPCR assay and confirmatory RNA sequencing to assess JCV prevalence in a vector candidate, Ochlerotatus canadensis. We identified JCV in 0.6% of mosquito pools, a similar prevalence to neighboring Connecticut. We assembled the first Massachusetts JCV genome directly from a mosquito sample, finding high identity to JCV isolates collected over a 60-year period. Further studies are needed to reconcile the low vector prevalence and low rate of viral evolutionary change with the increasing number of reported cases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2020.1756697 |
| URL الوصول: | https://doaj.org/article/c40367d46aa2489094bc6b641573f610 |
| رقم الأكسشن: | edsdoj.40367d46aa2489094bc6b641573f610 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2020.1756697 |