دورية أكاديمية
Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin
| العنوان: | Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin |
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| المؤلفون: | Caliandra Maria Bezerra Luna Lima, Francisca Inês de Sousa Freitas, Liana Clébia Soares Lima de Morais, Marília Gabriela dos Santos Cavalcanti, Lânia Ferreira da Silva, Rafael José Ribeiro Padilha, Constância Gayoso Simões Barbosa, Fábio André Brayner dos Santos, Luiz Carlos Alves, Margareth de Fátima Formiga Melo Diniz |
| المصدر: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 327-330 (2011) |
| بيانات النشر: | Sociedade Brasileira de Medicina Tropical (SBMT), 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine |
| مصطلحات موضوعية: | Alho, Schistosoma mansoni, Microscopia eletrônica de varredura, Arctic medicine. Tropical medicine, RC955-962 |
| الوصف: | INTRODUCTION: Garlic has a wide range of actions, including antibacterial, antiviral, antifungal, antiprotozoal and anthelmintic actions. This antiparasitic activity has been attributed to allicin, which is the main constituent of garlic. The present study aimed to investigate the in vitro activity of allicin on the tegument of adult Schistosoma mansoni worms using scanning electron microscopy. METHODS: Swiss Webster mice were infected with S. mansoni cercariae (100 per mouse) and sacrificed 50 days later to acquire the adult worms. These worms were collected by perfusion and placed in RPMI medium 1,640 at 37°C before transferring to RPMI media containing 0 (control), 5, 10, 15 and 20mg/mL of allicin, where they were incubated for 2h. The worms were fixed in 2.5% glutaraldehyde solution, washed twice, post-fixed in osmium tetroxide, washed twice and then dehydrated with ascending grades of ethanol. The samples were air-dried, mounted on stubs, gold coated in an ion sputtering unit and viewed using a scanning electron microscope. RESULTS: A concentration of 5mg/mL caused wrinkling in the tegument; a concentration of 10mg/mL resulted in changes to tubercles and loss or modification of spines. With 15 and 20mg/mL increasing damage to the tegument could be seen, such as vesicle formation and the presence of ulcers. CONCLUSIONS: These findings demonstrate the effect of allicin on adult S. mansoni worms and indicate that most of the changes occur at concentrations greater than that normally indicated for treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-9849 0037-8682 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300013&lng=en&tlng=en; https://doaj.org/toc/1678-9849 |
| DOI: | 10.1590/s0037-86822011005000023 |
| URL الوصول: | https://doaj.org/article/ec404fb16e84496fb6dd61dc758e7645 |
| رقم الأكسشن: | edsdoj.404fb16e84496fb6dd61dc758e7645 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16789849 00378682 |
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| DOI: | 10.1590/s0037-86822011005000023 |