دورية أكاديمية
Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays
| العنوان: | Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays |
|---|---|
| المؤلفون: | Li Kiang Tan, Wing Yan Wong, Hui Ting Yang, Roland G. Huber, Peter J. Bond, Lee Ching Ng, Sebastian Maurer-Stroh, Hapuarachchige Chanditha Hapuarachchi |
| المصدر: | Diagnostics, Vol 10, Iss 1, p 11 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | flavivirus, diagnostics, nonstructural protein 1, cross-reactivity, false positives, Medicine (General), R5-920 |
| الوصف: | Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2075-4418 07825455 |
| Relation: | https://www.mdpi.com/2075-4418/10/1/11; https://doaj.org/toc/2075-4418 |
| DOI: | 10.3390/diagnostics10010011 |
| URL الوصول: | https://doaj.org/article/405adef078254555869d4e50b6bf3b4d |
| رقم الأكسشن: | edsdoj.405adef078254555869d4e50b6bf3b4d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20754418 07825455 |
|---|---|
| DOI: | 10.3390/diagnostics10010011 |