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DC-SIGN of Largemouth Bass (Micropterus salmoides) Mediates Immune Functions against Aeromonas hydrophila through Collaboration with the TLR Signaling Pathway

التفاصيل البيبلوغرافية
العنوان: DC-SIGN of Largemouth Bass (Micropterus salmoides) Mediates Immune Functions against Aeromonas hydrophila through Collaboration with the TLR Signaling Pathway
المؤلفون: Mengmeng Huang, Jingwen Liu, Zhenzhen Yuan, Youxing Xu, Yang Guo, Shun Yang, Hui Fei
المصدر: International Journal of Molecular Sciences, Vol 25, Iss 9, p 5013 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Micropterus salmoides, C-type lectins, pattern recognition receptors, DC-SIGN, TLR signaling pathway, phagocytosis, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: C-type lectins in organisms play an important role in the process of innate immunity. In this study, a C-type lectin belonging to the DC-SIGN class of Micropterus salmoides was identified. MsDC-SIGN is classified as a type II transmembrane protein. The extracellular segment of MsDC-SIGN possesses a coiled-coil region and a carbohydrate recognition domain (CRD). The key amino acid motifs of the extracellular CRD of MsDC-SIGN in Ca2+-binding site 2 were EPN (Glu-Pro-Asn) and WYD (Trp-Tyr-Asp). MsDC-SIGN-CRD can bind to four pathogen-associated molecular patterns (PAMPs), including lipopolysaccharide (LPS), glucan, peptidoglycan (PGN), and mannan. Moreover, it can also bind to Gram-positive, Gram-negative bacteria, and fungi. Its CRD can agglutinate microbes and displays D-mannose and D-galactose binding specificity. MsDC-SIGN was distributed in seven tissues of the largemouth bass, among which the highest expression was observed in the liver, followed by the spleen and intestine. Additionally, MsDC-SIGN was present on the membrane of M. salmoides leukocytes, thereby augmenting the phagocytic activity against bacteria. In a subsequent investigation, the expression patterns of the MsDC-SIGN gene and key genes associated with the TLR signaling pathway (TLR4, NF-κB, and IL10) exhibited an up-regulated expression response to the stimulation of Aeromonas hydrophila. Furthermore, through RNA interference of MsDC-SIGN, the expression level of the DC-SIGN signaling pathway-related gene (RAF1) and key genes associated with the TLR signaling pathway (TLR4, NF-κB, and IL10) was decreased. Therefore, MsDC-SIGN plays a pivotal role in the immune defense against A. hydrophila by modulating the TLR signaling pathway.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 25095013
1422-0067
1661-6596
Relation: https://www.mdpi.com/1422-0067/25/9/5013; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms25095013
URL الوصول: https://doaj.org/article/407f15ae6d044e7b8ea7a7dc6f9d3512
رقم الأكسشن: edsdoj.407f15ae6d044e7b8ea7a7dc6f9d3512
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:25095013
14220067
16616596
DOI:10.3390/ijms25095013