دورية أكاديمية
Blood microbial signatures associated with mortality in patients with sepsis: A pilot study
| العنوان: | Blood microbial signatures associated with mortality in patients with sepsis: A pilot study |
|---|---|
| المؤلفون: | Huarong Chen, Weixin Liu, Olabisi Oluwabukola Coker, Na Qin, Hongyan Chen, Yifei Wang, Xiaodong Liu, Lin Zhang, Gordon Y.S. Choi, Wai Tat Wong, Czarina C.H. Leung, Lowell Ling, Mamie Hui, Tony Gin, Sunny Hei Wong, Matthew Tak Vai Chan, William Ka Kei Wu |
| المصدر: | Heliyon, Vol 10, Iss 8, Pp e29572- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Sepsis, Microorganism, Blood, Next-generation sequencing, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Sepsis is a life-threatening illness caused by the dysregulated host response to infection. Nevertheless, our current knowledge of the microbial landscape in the blood of septic patients is still limited. Next-generation sequencing (NGS) is a sensitive method to quantitatively characterize microbiomes at various sites of the human body. In this study, we analyzed the blood microbial DNA of 22 adult patients with sepsis and 3 healthy subjects. The presence of non-human DNA was identified in both healthy and septic subjects. Septic patients had a markedly altered microbial DNA profile compared to healthy subjects over α- and β-diversity. Unexpectedly, the patients could be further divided into two subgroups (C1 and C2) based on β-diversity analysis. C1 patients showed much higher bacteria, viruses, fungi, and archaea abundance, and a higher level of α-diversity (Chao1, Observed and Shannon index) than both C2 patients and healthy subjects. The most striking difference was seen in the case of Streptomyces violaceusniger, Phenylobacterium sp. HYN0004, Caulobacter flavus, Streptomyces sp. 11-1-2, and Phenylobacterium zucineum, the abundance of which was the highest in the C1 group. Notably, C1 patients had a significantly poorer outcome than C2 patients. Moreover, by analyzing the patterns of microbe-microbe interactions in healthy and septic subjects, we revealed that C1 and C2 patients exhibited distinct co-occurrence and co-exclusion relationships. Together, our study uncovered two distinct microbial signatures in the blood of septic patients. Compositional and ecological analysis of blood microbial DNA may thus be useful in predicting mortality of septic patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844024056032; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2024.e29572 |
| URL الوصول: | https://doaj.org/article/40a38ee7d9394702a79e74e2ab959e34 |
| رقم الأكسشن: | edsdoj.40a38ee7d9394702a79e74e2ab959e34 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
|---|---|
| DOI: | 10.1016/j.heliyon.2024.e29572 |