دورية أكاديمية
Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
العنوان: | Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae) |
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المؤلفون: | Thiago Henrique Nascimento, Raquel S. Gonçalves, Mariana Báez, Guillermo Seijo, Marcelo Guerra |
المصدر: | Boletín de la Sociedad Argentina de Botánica, Vol 57, Iss 1 (2022) |
بيانات النشر: | Sociedad Argentina de Botánica, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Science LCC:Botany |
مصطلحات موضوعية: | CMA/DAPI banding, cytotaxonomy, FISH, monotrisomy, rDNA, telomeric DNA, Science, Botany, QK1-989 |
الوصف: | Background and aims: Zephyranthes brachyandra belongs to a tribe of ornamental Amaryllidaceae native of South America, whose genera circumscription and phylogenetic relationships are still unclear. Cytologically, Z. brachyandra is a tetraploid whose chromosomes are of similar size and morphology, hindering the identification of its 2n = 24 chromosomes. The aim of this study was to investigate the stability of the many CMA+ and DAPI+ bands and the occurrence of B chromosomes by a cytomolecular approach. M&M: For this investigation we conducted a cytomolecular analysis with CMA/DAPI staining and fluorescence in situ hybridization with 5S and 35S rDNA probes, and the TTTAGGG telomeric probe. Results: In the present work, a cytomolecular analysis of Z. brachyandra, revealed a large and variable number of CMA+ and DAPI+ heterochromatic bands and 5S and 35S rDNA sites, and a regular distribution of the TTTAGGG telomeric sequences. In addition, one individual was monotrisomic with 2n = 24, and another one had a B chromosome. Both numerical and structural chromosome alterations were clearly characterized by CMA/DAPI bands and rDNA sites. Conclusions: Comparing the present data with the cytological data for other species of Zephyranthes, it becomes clear that a cytomolecular approach is fundamental to the understanding of the chromosome variation and cytotaxonomy of the group. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English Spanish; Castilian Portuguese |
تدمد: | 0373-580X 1851-2372 |
Relation: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304; https://doaj.org/toc/0373-580X; https://doaj.org/toc/1851-2372 |
URL الوصول: | https://doaj.org/article/deac416cecaf47219ab2641e9e15e52f |
رقم الأكسشن: | edsdoj.416cecaf47219ab2641e9e15e52f |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 0373580X 18512372 |
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