دورية أكاديمية
LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis
العنوان: | LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis |
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المؤلفون: | XueFeng Sun, GuangSuo Wang, PeiKun Ding, ShiXuan Li |
المصدر: | Brazilian Journal of Medical and Biological Research, Vol 53, Iss 12 (2020) |
بيانات النشر: | Associação Brasileira de Divulgação Científica, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
مصطلحات موضوعية: | LINC00355, miR-466, LYAR, ceRNA, Lung squamous cell carcinoma, Medicine (General), R5-920, Biology (General), QH301-705.5 |
الوصف: | LINC00355 has been reported aberrantly over-expressed and associated with poor prognosis in various types of cancer. However, reports regarding the effect of LINC00355 on lung squamous cell carcinoma (SCC) are rare. This study aimed to explore the function of LINC00355 in the development and progression of lung SCC and reveal the underlying mechanism. The expression and subcellular location of LINC00355 were determined by qRT-PCR and RNA-FISH, respectively. The lung SCC cell growth was analyzed by CCK-8 assay, transwell invasion, wound healing, colony formation, and flow cytometry assays. Reactive oxygen species level was evaluated by DCFH-DA probes. Bioinformatics online websites, luciferase reporter assay, RNA binding protein immunoprecipitation (RIP), and RNA pull-down assays were utilized to investigate the interaction among LINC00355, miR-466, and Ly-1 antibody reactive clone (LYAR). The results showed that LINC00355 was upregulated in lung SCC and was positively associated with poor overall survival in lung SCC patients. LINC00355 was mainly located in the cytoplasm of SCC cells. Additionally, LINC0035 functioned as a competing endogenous RNA (ceRNA) to target miR-466, and LYAR was identified as a direct target of miR-466. LINC00355 expression negatively correlated with miR-466 level, and positively correlated with LYAR level. Mechanistically, knockdown of LINC00355 inhibited cell proliferation, migration and invasion, promoted cell apoptosis in vitro, and suppressed tumor growth in vivo through targeting miR-466, and thus down-regulated LYAR expression. These findings provide a new sight for understanding the molecular mechanism of lung SCC and indicate that LINC00355 may serve as a potential biomarker for the diagnosis and treatment of lung SCC. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1414-431X 1414-431x |
Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020001200606&tlng=en; http://www.scielo.br/pdf/bjmbr/v53n12/1414-431X-bjmbr-53-12-e9317.pdf; https://doaj.org/toc/1414-431X |
DOI: | 10.1590/1414-431x20209317 |
URL الوصول: | https://doaj.org/article/4192af099a084b529237ae6cb5abd8cd |
رقم الأكسشن: | edsdoj.4192af099a084b529237ae6cb5abd8cd |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1414431X 1414431x |
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DOI: | 10.1590/1414-431x20209317 |