دورية أكاديمية
Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China
| العنوان: | Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China |
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| المؤلفون: | Qiao Mengfan, Wang Lixia, Lei Ying, Ren Yan, Cai Kuojun, Zhang Jinsheng, Zhang Zaichao, Yu Weiwei, Peng Yelong, Cai Xuepeng, Li Chongyang, Qiao Jun, Meng Qingling |
| المصدر: | Veterinary World, Vol 13, Iss 5, Pp 916-922 (2020) |
| بيانات النشر: | Veterinary World, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | ehrlichia canis, genetic characteristics, gp36, pet dog, rhipicephalus sanguineus sensu lato, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0972-8988 2231-0916 |
| Relation: | http://www.veterinaryworld.org/Vol.13/May-2020/13.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2020.916-922 |
| URL الوصول: | https://doaj.org/article/a41f531a1369437ea3dc8f09ff24ea04 |
| رقم الأكسشن: | edsdoj.41f531a1369437ea3dc8f09ff24ea04 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2020.916-922 |