دورية أكاديمية
Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomics
| العنوان: | Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomics |
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| المؤلفون: | Mani D R, Abbatiello Susan E, Carr Steven A |
| المصدر: | BMC Bioinformatics, Vol 13, Iss Suppl 16, p S9 (2012) |
| بيانات النشر: | BMC, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Computer applications to medicine. Medical informatics LCC:Biology (General) |
| مصطلحات موضوعية: | Multiple reaction monitoring mass spectrometry (MRM-MS), stable isotope dilution (SID), quantification, interference detection, limits of detection and quantification, intra- and interlaboratory precision, Computer applications to medicine. Medical informatics, R858-859.7, Biology (General), QH301-705.5 |
| الوصف: | Abstract Multiple reaction monitoring mass spectrometry (MRM-MS) with stable isotope dilution (SID) is increasingly becoming a widely accepted assay for the quantification of proteins and peptides. These assays have shown great promise in relatively high throughput verification of candidate biomarkers. While the use of MRM-MS assays is well established in the small molecule realm, their introduction and use in proteomics is relatively recent. As such, statistical and computational methods for the analysis of MRM-MS data from proteins and peptides are still being developed. Based on our extensive experience with analyzing a wide range of SID-MRM-MS data, we set forth a methodology for analysis that encompasses significant aspects ranging from data quality assessment, assay characterization including calibration curves, limits of detection (LOD) and quantification (LOQ), and measurement of intra- and interlaboratory precision. We draw upon publicly available seminal datasets to illustrate our methods and algorithms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2105 75997444 |
| Relation: | http://www.biomedcentral.com/1471-2105/13/S16/S9; https://doaj.org/toc/1471-2105 |
| DOI: | 10.1186/1471-2105-13-S16-S9 |
| URL الوصول: | https://doaj.org/article/c421f1f75997444d93c4dd2aa3abf9a1 |
| رقم الأكسشن: | edsdoj.421f1f75997444d93c4dd2aa3abf9a1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712105 75997444 |
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| DOI: | 10.1186/1471-2105-13-S16-S9 |