دورية أكاديمية
In vivo effects of renal Npt2a inhibition
| العنوان: | In vivo effects of renal Npt2a inhibition |
|---|---|
| المؤلفون: | Timo Rieg, Linto Thomas, Jianxiang Xue, Jessica Ann Dominguez Rieg |
| المصدر: | Revista Cubana de Investigaciones Biomédicas, Vol 38, Iss 5 (2020) |
| بيانات النشر: | Centro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED), 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | npt2a inhibitor, mice, urinary phosphate excretion, animal model, mice, Medicine (General), R5-920 |
| الوصف: | Introduction: Hyperphosphatemia is common in patients with chronic kidney disease and associated with increased mortality. Oral phosphate binders and dietary phosphate restriction are the current management protocols for patients with hyperphosphatemia; however, their effectiveness is insufficient. In the kidney, the sodium-phosphate cotransporter Npt2a is responsible for bulk uptake of phosphate in the proximal tubule. Recently, an orally bioavailable selective Npt2a inhibitor (Npt2a-I, PF-06869206) has been described to reduce phosphate uptake in HEK cells transfected with mouse or rat Npt2a. So far, its physiological in vivo function has not been tested. Objective: To describe the in vivo effect of renal NPt2a inhibition in C57BL/6J mice Material and Methods: Based on in vitro IC 50 concentrations, we chose to study 30 mg/kg (oral gavage, 1% of body weight) in short-term (3 hours) metabolic cage experiments in C57BL/6J mice. Results: Compared to vehicle (n=14), bolus administration of Npt2a-I (n=12) caused significantly higher (~4-fold) urinary phosphate excretion (104±8 vs 27 ±6 µmol*min -1, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Spanish; Castilian |
| تدمد: | 0864-0300 1561-3011 |
| Relation: | http://www.revibiomedica.sld.cu/index.php/ibi/article/view/485; https://doaj.org/toc/0864-0300; https://doaj.org/toc/1561-3011 |
| URL الوصول: | https://doaj.org/article/dd428a15acb743f29849df37913b355d |
| رقم الأكسشن: | edsdoj.428a15acb743f29849df37913b355d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 08640300 15613011 |
|---|