دورية أكاديمية
RNA-Seq analysis of the fruiting bodies and mycelia of angel-wing mushroom Pleurocybella porrigens that cause acute encephalopathy
| العنوان: | RNA-Seq analysis of the fruiting bodies and mycelia of angel-wing mushroom Pleurocybella porrigens that cause acute encephalopathy |
|---|---|
| المؤلفون: | Nozomu Watanabe, Keisuke Mitsukuni, Takumi Sato, Jili Zhang, Akiko Ono, Tomohiro Suzuki |
| المصدر: | BMC Research Notes, Vol 17, Iss 1, Pp 1-7 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| مصطلحات موضوعية: | Fungi, Gene expression analysis, Lectin, Real-time RT-PCR, Transcriptome, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| الوصف: | Abstract Objective In 2004, after consuming angel-wing mushrooms, Pleurocybella porrigens, 59 incidents of food poisoning were reported in Japan. Consequently, 17 individuals died of acute encephalopathy. In 2023, we proved that a lectin, pleurocybelline, and pleurocybellaziridine from this mushroom caused damage to the brains of mice. Although we reported genomic and transcriptomic data of P. porrigens in 2013, the assembly quality of the transcriptomic data was inadequate for accurate functional annotation. Thus, we obtained detailed transcriptomic data on the fruiting bodies and mycelia of this mushroom using Illumina NovaSeq 6000. Results De novo assembly data indicated that the N50 lengths for the fruiting bodies and mycelia were improved compared with those previously reported. The differential expression analysis between the fruiting bodies and the mycelia revealed that 1,937 and 1,555 genes were significantly up-regulated in the fruiting bodies and the mycelia, respectively. The biological functions of P. porrigens transcripts, including PA biosynthetic pathways, were investigated using BLAST search, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The obtained results revealed L-valine, a predicted precursor of PA, is biosynthesized in the fruiting bodies and mycelia. Furthermore, real-time RT-PCR was performed to evaluate the accuracy of the results of differential expression analysis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-0500 |
| Relation: | https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-024-06860-2 |
| URL الوصول: | https://doaj.org/article/42a5989368f348a8af8cd9c4ca259078 |
| رقم الأكسشن: | edsdoj.42a5989368f348a8af8cd9c4ca259078 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17560500 |
|---|---|
| DOI: | 10.1186/s13104-024-06860-2 |