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Influenza A Infection Stimulates RIG-I and Enhances Effector Function of Primary Human NK Cells

التفاصيل البيبلوغرافية
العنوان: Influenza A Infection Stimulates RIG-I and Enhances Effector Function of Primary Human NK Cells
المؤلفون: Adham Abuelola Mohamed, Sofía Soler, Julia Wegner, Eva Bartok, Sanda Stankovic, Andrew G. Brooks, Martin Schlee
المصدر: International Journal of Molecular Sciences, Vol 24, Iss 15, p 12220 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Influenza A, NK cells, RIG-I, IFN-α/β, innate nucleic acid receptors, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Immune surveillance by natural killer (NK) cells and their recruitment to sites of inflammation renders them susceptible to viral infection, potentially modulating their effector function. Here, we analyzed innate RNA receptor signaling in NK cells downstream of direct Influenza A virus (IAV) infection and its impact on NK cell effector function. Infection of NK cells with IAV resulted in the activation of TBK1, NF-ϰB and subsequent type-I IFN secretion. CRISPR-generated knockouts in primary human NK cells revealed that this effect depended on the antiviral cytosolic RNA receptor RIG-I. Transfection of NK cells with synthetic 3p-dsRNA, a strong RIG-I agonist that mimics viral RNA, resulted in a similar phenotype and rendered NK cells resistant to subsequent IAV infection. Strikingly, both IAV infection and 3p-dsRNA transfection enhanced degranulation and cytokine production by NK cells when exposed to target cells. Thus, RIG-I activation in NK cells both supports their cell intrinsic viral defense and enhances their cytotoxic effector function against target cells.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
1661-6596
Relation: https://www.mdpi.com/1422-0067/24/15/12220; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms241512220
URL الوصول: https://doaj.org/article/c42af2fea2934a43a0ee01234fb10506
رقم الأكسشن: edsdoj.42af2fea2934a43a0ee01234fb10506
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
16616596
DOI:10.3390/ijms241512220