دورية أكاديمية
Modelling invasive group A streptococcal disease using bioluminescence
| العنوان: | Modelling invasive group A streptococcal disease using bioluminescence |
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| المؤلفون: | L. E. Lamb, X. Zhi, F. Alam, M. Pyzio, C. L. Scudamore, S. Wiles, S. Sriskandan |
| المصدر: | BMC Microbiology, Vol 18, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | Bioluminescence, Biophotonic imaging, Group A Streptococcus, Infection model, Invasive disease, Luciferase, Microbiology, QR1-502 |
| الوصف: | Abstract Background The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in infection modelling and refine the information that can be obtained, however the range of bioluminescent GAS strains available is limited. In this study we set out to develop bioluminescent iGAS strains for use in in vivo pneumonia and soft tissue disease models. Results Using clinical emm1, emm3, and emm89 GAS strains that were transformed with constructs carrying the luxABCDE operon, growth and bioluminescence of transformed strains were characterised in vitro and in vivo. Emm3 and emm89 strains expressed detectable bioluminescence when transformed with a replicating plasmid and light production correlated with viable bacterial counts in vitro, however plasmid instability precluded use in the absence of antimicrobial pressure. Emm89 GAS transformed with an integrating construct demonstrated stable bioluminescence that was maintained in the absence of antibiotics. Bioluminescence of the emm89 strain correlated with viable bacterial counts both in vitro and immediately following infection in vivo. Although bioluminescence conferred a detectable fitness burden to the emm89 strain during soft tissue infection in vivo, it did not prevent dissemination to distant tissues. Conclusion Development of stably bioluminescent GAS for use in vitro and in vivo models of infection should facilitate development of novel therapeutics and vaccines while also increasing our understanding of infection progression and transmission routes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2180 89600754 |
| Relation: | http://link.springer.com/article/10.1186/s12866-018-1200-1; https://doaj.org/toc/1471-2180 |
| DOI: | 10.1186/s12866-018-1200-1 |
| URL الوصول: | https://doaj.org/article/ea43b8960075495cb699cb7e456eddd3 |
| رقم الأكسشن: | edsdoj.43b8960075495cb699cb7e456eddd3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712180 89600754 |
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| DOI: | 10.1186/s12866-018-1200-1 |