دورية أكاديمية
Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein
| العنوان: | Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein |
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| المؤلفون: | Zhan Qin, Shi Changhua, Jiang Yu, Gao Xianling, Lin Ying |
| المصدر: | Acta Biochimica et Biophysica Sinica, Vol 55, Pp 1310-1318 (2023) |
| بيانات النشر: | China Science Publishing & Media Ltd., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biochemistry LCC:Genetics |
| مصطلحات موضوعية: | Cne PRP8 intein, C-extein, directed evolution, Biochemistry, QD415-436, Genetics, QH426-470 |
| الوصف: | Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE intein obtained from the directed evolution of Cne PRP8, which shows that its splicing rate is ~29- fold higher than that of the wild-type. When the +1 residue of C-extein is changed to cysteine, CPE also shows high splicing activity. Faster association and higher affinity may contribute to the high splicing rate compared with wild-type intein. These findings have important implications for the future engineering of inteins and provide clues for fundamental studies of protein structure and folding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1672-9145 |
| Relation: | https://doaj.org/toc/1672-9145 |
| DOI: | 10.3724/abbs.2023135 |
| URL الوصول: | https://doaj.org/article/43da6aee4ede4527af47a561d51ab8fb |
| رقم الأكسشن: | edsdoj.43da6aee4ede4527af47a561d51ab8fb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16729145 |
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| DOI: | 10.3724/abbs.2023135 |