دورية أكاديمية
Comparative assessment of embryotoxicity of 2,4,6-triiodophenol to mouse blastoid and pre-implantation embryo models
| العنوان: | Comparative assessment of embryotoxicity of 2,4,6-triiodophenol to mouse blastoid and pre-implantation embryo models |
|---|---|
| المؤلفون: | Xiaoqian Zhang, Shiyu An, Siya Liu, Jingfan Qiu, Wenyi Zhang, Qing Zhou, Xiaojing Hou, Yang Yang |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 252, Iss , Pp 114608- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | 2,4,6-triiodophenol, Embryotoxicity, Blastoid model, Mouse preimplantation embryo model, Reliability assessment, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | Embryonic developmental effects of disinfection by-products, which are generated during drinking water treatment and widely detected in environment, have gained more and more attention nowadays, calling for construction of in vitro research models which can mimic early embryonic development to evaluate the embryotoxicity. The embryonic stem cell test offers a promising assay to predict embryotoxicity of environmental pollutions. However, it is not appropriate for the toxicological study of preimplantation embryos. Here, we used mouse extended stem cells (mEPS) to reconstruct embryo-like structures (blastoid), furtherly attempting to evaluate the reliability of this model for the prediction of possible developmental toxicity of 2,4,6-triiodophenol (TIP, 5–50 μM), a novel halogenated disinfection byproduct widely detected in water and even drinking water, to mammalian preimplantation embryo. To verify this, we treated mouse embryo derived from in vitro fertilization (IVF-embryo) as reference. The results showed that mEPS-blastoid was like natural blastocyst in morphology, cell composition, and could recapitulate key developmental events happened during mouse preimplantation stage. When blastoid and IVF-embryo models were separately exposed to TIP, their final blastocyst formation rates were not impaired, according to morphological features, meanwhile that TIP exposure caused slight cell apoptosis. Besides, TIP induced an ICM cell bias in cell fate decision, resulting in cell proportion change, which implied abnormal developmental potential. Though we could not evaluate TIP’s embryotoxicity before 8-cell stage using blastoid model, its viability as a novel and high-throughput assessment platform for increasing environmental pollutants was still recognized. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651323001124; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2023.114608 |
| URL الوصول: | https://doaj.org/article/4457e11af6d6481d91c73d2334867e51 |
| رقم الأكسشن: | edsdoj.4457e11af6d6481d91c73d2334867e51 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 |
|---|---|
| DOI: | 10.1016/j.ecoenv.2023.114608 |