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Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth

التفاصيل البيبلوغرافية
العنوان: Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth
المؤلفون: Karla Marie Márquez-Nogueras, Miryam Andrea Hortua Triana, Nathan M Chasen, Ivana Y Kuo, Silvia NJ Moreno
المصدر: eLife, Vol 10 (2021)
بيانات النشر: eLife Sciences Publications Ltd, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Toxoplasma gondii, calcium signaling, transient receptor channel, calcium entry, ER calcium, Medicine, Science, Biology (General), QH301-705.5
الوصف: Transient receptor potential (TRP) channels participate in calcium ion (Ca2+) influx and intracellular Ca2+ release. TRP channels have not been studied in Toxoplasma gondii or any other apicomplexan parasite. In this work, we characterize TgGT1_310560, a protein predicted to possess a TRP domain (TgTRPPL-2), and determined its role in Ca2+ signaling in T. gondii, the causative agent of toxoplasmosis. TgTRPPL-2 localizes to the plasma membrane and the endoplasmic reticulum (ER) of T. gondii. The ΔTgTRPPL-2 mutant was defective in growth and cytosolic Ca2+ influx from both extracellular and intracellular sources. Heterologous expression of TgTRPPL-2 in HEK-3KO cells allowed its functional characterization. Patching of ER-nuclear membranes demonstrates that TgTRPPL-2 is a non-selective cation channel that conducts Ca2+. Pharmacological blockers of TgTRPPL-2 inhibit Ca2+ influx and parasite growth. This is the first report of an apicomplexan ion channel that conducts Ca2+ and may initiate a Ca2+ signaling cascade that leads to the stimulation of motility, invasion, and egress. TgTRPPL-2 is a potential target for combating toxoplasmosis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/63417; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.63417
URL الوصول: https://doaj.org/article/447eb7e63c784937a8a63cc25a658255
رقم الأكسشن: edsdoj.447eb7e63c784937a8a63cc25a658255
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.63417