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Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.

التفاصيل البيبلوغرافية
العنوان: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.
المؤلفون: C Mendoza-Topaz, G Nelson, G Howard, S Hafner, P Rademacher, M Frick, B J Nichols
المصدر: PLoS ONE, Vol 13, Iss 10, p e0205306 (2018)
بيانات النشر: Public Library of Science (PLoS), 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC6197626?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0205306
URL الوصول: https://doaj.org/article/44a7a31a540b495fb8918556185614d8
رقم الأكسشن: edsdoj.44a7a31a540b495fb8918556185614d8
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0205306