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DNA Barcoding Technology Used to Successfully Sub-Classify a Museum Whale Specimen as Balaenoptera edeni edeni

التفاصيل البيبلوغرافية
العنوان: DNA Barcoding Technology Used to Successfully Sub-Classify a Museum Whale Specimen as Balaenoptera edeni edeni
المؤلفون: Xiaoying Ren, Xiaolin Ma, Edward Allen, Yuan Fang, Shaoqing Wen
المصدر: Frontiers in Ecology and Evolution, Vol 10 (2022)
بيانات النشر: Frontiers Media S.A., 2022.
سنة النشر: 2022
المجموعة: LCC:Evolution
LCC:Ecology
مصطلحات موضوعية: museum specimen, Bryde’s whale, morphological identification, DNA barcoding, DNA identification, Evolution, QH359-425, Ecology, QH540-549.5
الوصف: DNA barcoding technology is becoming an increasingly powerful tool in resolving issues of detailed species identification based on morphology, as commonly employed by museums. In the present study, we aimed to identify a stranded Bryde’s whale on Hainan Island, China by extracting DNA from a vertebra pre-treated by physical and/or chemical processes. Based on morphological characteristics, this Bryde’s whale was initially determined as Balaenoptera edeni. Then, DNA was efficiently extracted using ancient DNA techniques. The mitochondrial gene (COI) phylogenetic analysis further revealed that this museum whale specimen belonged to the sub-species B. e. edeni. This study provides a testable and rapid method for museum species verification, by using ancient DNA extraction methods to compensate the disadvantage of traditional DNA extraction methods that are difficult to extract valid DNA.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2296-701X
Relation: https://www.frontiersin.org/articles/10.3389/fevo.2022.921106/full; https://doaj.org/toc/2296-701X
DOI: 10.3389/fevo.2022.921106
URL الوصول: https://doaj.org/article/c44d9bd2193f41159a522a40c3525fd4
رقم الأكسشن: edsdoj.44d9bd2193f41159a522a40c3525fd4
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2296701X
DOI:10.3389/fevo.2022.921106