دورية أكاديمية
Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs
| العنوان: | Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs |
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| المؤلفون: | Mieczysław Sajewicz, Agata Kabała-Dzik, Joanna Smoleń-Dzirba, Jerzy Stojko, Robert Kubina, Małgorzata Kępa, Danuta Idzik, Robert D. Wojtyczka, Arkadiusz Dziedzic, Tomasz J. Wąsik |
| المصدر: | Molecules, Vol 18, Iss 8, Pp 9623-9640 (2013) |
| بيانات النشر: | MDPI AG, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | methicillin-resistant S. aureus (MRSA), methicillin-sensitive S. aureus (MSSA), ethanolic extract of Polish propolis (EEPP), Organic chemistry, QD241-441 |
| الوصف: | The objective of this study was to assess in vitro the antimicrobial activity of ethanolic extract of Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. The combined effect of EEPP and 10 selected antistaphylococcal drugs on S. aureus clinical cultures was also investigated. EEPP composition was analyzed by a High Performance Liquid Chromatography (HPLC) method. The flavonoid compounds identified in Polish Propolis included flavones, flavonones, flavonolols, flavonols and phenolic acids. EEPP displayed varying effectiveness against twelve S. aureus strains, with minimal inhibitory concentration (MIC) within the range from 0.39 to 0.78 mg/mL, determined by broth microdilution method. The average MIC was 0.54 ± 0.22 mg/mL, while calculated MIC50 and MIC90 were 0.39 mg/mL and 0.78 mg/mL, respectively. The minimum bactericidal concentration (MBC) of the EEPP ranged from 0.78 to 3.13 mg/mL. The in vitro combined effect of EEPP and 10 antibacterial drugs was investigated using disk diffusion method-based assay. Addition of EEPP to cefoxitin (FOX), clindamycin (DA), tetracycline (TE), tobramycin (TOB), linezolid (LIN), trimethoprim+sulfamethoxazole (SXT), penicillin (P), erythromycin (E) regimen, yielded stronger, cumulative antimicrobial effect, against all tested S. aureus strains than EEPP and chemotherapeutics alone. In the case of ciprofloxacin (CIP) and chloramphenicol (C) no synergism with EEPP was observed. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 18089623 1420-3049 |
| Relation: | http://www.mdpi.com/1420-3049/18/8/9623; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules18089623 |
| URL الوصول: | https://doaj.org/article/a450035a25784a8f80c8fdbf837acdb8 |
| رقم الأكسشن: | edsdoj.450035a25784a8f80c8fdbf837acdb8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18089623 14203049 |
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| DOI: | 10.3390/molecules18089623 |