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Production of genome-edited Daphnia for heavy metal detection by fluorescence

التفاصيل البيبلوغرافية
العنوان: Production of genome-edited Daphnia for heavy metal detection by fluorescence
المؤلفون: Takuto Arao, Yasuhiko Kato, Quang Dang Nong, Hiroshi Yamamoto, Haruna Watanabe, Tomoaki Matsuura, Norihisa Tatarazako, Kazune Tani, Akira Okamoto, Takeru Matsumoto, Hajime Watanabe
المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
بيانات النشر: Nature Portfolio, 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from Daphnia magna. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into D. magna using the TALEN technique and transgenic Daphnia named D. magna MetalloG were produced. When D. magna MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn2+, 130 nM Cu2+, and 70 nM Cd2+. Heavy metal exposure for 24 h could lower the thresholds even further. D. magna MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-020-78572-z
URL الوصول: https://doaj.org/article/4582b086e72940e087dff3c8bafedcea
رقم الأكسشن: edsdoj.4582b086e72940e087dff3c8bafedcea
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-020-78572-z