This study was carried out to determine the effects of healthy lifestyle behaviors on the COVID-19 vaccine attitude in the Z generation. The descriptive cross-sectional study was conducted in Turkey using web-based questionnaires, and 510 participants (348 women, 162 men, age 15-24) in the Z generation. The data were collected by using Personal Information Form and Health Lifestyle Behaviors Scale-II (HLBS-II). It was determined that 89.4% of the female participants and 85.8% of the male participants, who participated in the study, had the COVID-19 vaccine. It was determined that 84.2% of the women who had the COVID-19 vaccine received the second dose, while 76.3% of the men had the second dose. It was determined that the mean scores of women and men who had COVID-19 vaccine, found the COVID-19 vaccines safe, and believed that it was more beneficial than harm, had higher mean scores from HLBS-II and the difference between the groups was statistically significant (p [Med-Science 2022; 11(1.000): 53-8]