دورية أكاديمية
Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant
| العنوان: | Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant |
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| المؤلفون: | Amit Bhangale, Amardeep Pathak, Smita Pawar, Tarun Jeloka |
| المصدر: | Asian Journal of Transfusion Science, Vol 11, Iss 2, Pp 131-134 (2017) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: | ABO incompatible kidney transplants, column agglutination technique, conventional tube technique, Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: | Introduction: Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). Material and Methods: There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the two methods so as to set the 'cut-off' titer accordingly, depending upon the method used. Results: We did a prospective observational study to compare and correlate the ABO titers using these two different techniques – conventional tube technique (CTT) and the newer column agglutination technique (CAT). A total of 67 samples were processed in parallel for anti-A/B antibodies by both tube dilution and column agglutination methods. The mean titer by conventional tube method was 38.5 + 96.6 and by the column agglutination test was 96.4 + 225. The samples correlated well with Spearman rho correlation coefficient of 0.94 (P = 0.01). Conclusion: The column agglutination method for anti A/B titer estimation in an ABO incompatible kidney transplant is more sensitive, with the column agglutination results being approximately two and half fold higher (one more dilution) than that of tube method. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0973-6247 1998-3565 |
| Relation: | http://www.ajts.org/article.asp?issn=0973-6247;year=2017;volume=11;issue=2;spage=131;epage=134;aulast=Bhangale; https://doaj.org/toc/0973-6247; https://doaj.org/toc/1998-3565 |
| DOI: | 10.4103/0973-6247.214343 |
| URL الوصول: | https://doaj.org/article/4667b8b30fc9457e803cf86cb2055df9 |
| رقم الأكسشن: | edsdoj.4667b8b30fc9457e803cf86cb2055df9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09736247 19983565 |
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| DOI: | 10.4103/0973-6247.214343 |