دورية أكاديمية
Efficient and versatile multiplex prime editing in hexaploid wheat
العنوان: | Efficient and versatile multiplex prime editing in hexaploid wheat |
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المؤلفون: | Pei Ni, Yidi Zhao, Ximeng Zhou, Zehua Liu, Zhengwei Huang, Zhongfu Ni, Qixin Sun, Yuan Zong |
المصدر: | Genome Biology, Vol 24, Iss 1, Pp 1-19 (2023) |
بيانات النشر: | BMC, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Biology (General) LCC:Genetics |
مصطلحات موضوعية: | Hexaploid wheat, Prime editing, Reverse transcriptase, Multiplex genome editing, Biology (General), QH301-705.5, Genetics, QH426-470 |
الوصف: | Abstract Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fold and 6.4-fold compared to the original PPE and ePPE, respectively. Importantly, a robust multiplex prime editing platform is established for simultaneous editing of four to ten genes in protoplasts and up to eight genes in regenerated wheat plants at frequencies up to 74.5%, thus expanding the applicability of prime editors for stacking of multiple agronomic traits. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1474-760X |
Relation: | https://doaj.org/toc/1474-760X |
DOI: | 10.1186/s13059-023-02990-1 |
URL الوصول: | https://doaj.org/article/4682f652a27d469080bf348218d7cd35 |
رقم الأكسشن: | edsdoj.4682f652a27d469080bf348218d7cd35 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1474760X |
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DOI: | 10.1186/s13059-023-02990-1 |