دورية أكاديمية

BEtarget: A versatile web-based tool to design guide RNAs for base editing in plants

التفاصيل البيبلوغرافية
العنوان: BEtarget: A versatile web-based tool to design guide RNAs for base editing in plants
المؤلفون: Xianrong Xie, Fuquan Li, Xiyu Tan, Dongchang Zeng, Weizhi Liu, Wanyong Zeng, Qinlong Zhu, Yao-Guang Liu
المصدر: Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 4009-4014 (2022)
بيانات النشر: Elsevier, 2022.
سنة النشر: 2022
المجموعة: LCC:Biotechnology
مصطلحات موضوعية: CRISPR, Base editing, gRNA design, Genome editing, Biotechnology, TP248.13-248.65
الوصف: CRISPR-dependent base editors enable direct nucleotide conversion without the introduction of double-strand DNA break or donor DNA template, thus expanding the CRISPR toolbox for genetic manipulation. However, designing guide RNAs (gRNAs) for base editors to enable gene correction or inactivation is more complicated than using the CRISPR system for gene disruption. Here, we present a user-friendly web tool named BEtarget dedicated to the design of gRNA for base editing. It is currently supported by 46 plant reference genomes and 5 genomes of non-plant model organisms. BEtarget supports the design of gRNAs with different types of protospacer adjacent motifs (PAM) and integrates various functions, including automatic identification of open reading frame, prediction of potential off-target sites, annotation of codon change, and assessment of gRNA quality. Moreover, the program provides an interactive interface for users to selectively display information about the desired target sites. In brief, we have developed a flexible and versatile web-based tool to simplify complications associated with the design of base editing technology. BEtarget is freely accessible at https://skl.scau.edu.cn/betarget/.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2001-0370
Relation: http://www.sciencedirect.com/science/article/pii/S2001037022003269; https://doaj.org/toc/2001-0370
DOI: 10.1016/j.csbj.2022.07.046
URL الوصول: https://doaj.org/article/46ab17f4488f4e5b8dcc634fa9cf989d
رقم الأكسشن: edsdoj.46ab17f4488f4e5b8dcc634fa9cf989d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20010370
DOI:10.1016/j.csbj.2022.07.046