دورية أكاديمية
Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses with Acute Abdominal Disease
| العنوان: | Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses with Acute Abdominal Disease |
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| المؤلفون: | Alberto Muñoz-Prieto, Damián Escribano, María Dolores Contreras-Aguilar, Anita Horvatić, Nicolas Guillemin, Stine Jacobsen, José Joaquín Cerón, Vladimir Mrljak |
| المصدر: | Animals, Vol 11, Iss 5, p 1304 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | tandem mass tag, proteomic, saliva, horses, acute abdominal disease, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the proteomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregulation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, especially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/11/5/1304; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani11051304 |
| URL الوصول: | https://doaj.org/article/46c17f48ecfa45e5b4055234b77e4b8f |
| رقم الأكسشن: | edsdoj.46c17f48ecfa45e5b4055234b77e4b8f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20762615 |
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| DOI: | 10.3390/ani11051304 |