دورية أكاديمية
Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection
| العنوان: | Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection |
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| المؤلفون: | Dominik Nörz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, Marc Lütgehetmann |
| المصدر: | Emerging Infectious Diseases, Vol 28, Iss 9, Pp 1765-1769 (2022) |
| بيانات النشر: | Centers for Disease Control and Prevention, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | monkeypox virus, viruses, zoonoses, SARS-CoV-2, molecular diagnostics, high-throughput PCR testing, Medicine, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Beginning in May 2022, a rising number of monkeypox cases were reported in non–monkeypox-endemic countries in the Northern Hemisphere. We adapted 2 published quantitative PCRs for use as a dual-target monkeypox virus test on widely used automated high-throughput PCR systems. We determined analytic performance by serial dilutions of monkeypox virus reference material, which we quantified by digital PCR. We found the lower limit of detection for the combined assays was 4.795 (95% CI 3.6–8.6) copies/mL. We compared clinical performance against a commercial manual orthopoxvirus research use only PCR kit by using clinical remnant swab samples. Our assay showed 100% positive (n = 11) and 100% negative (n = 56) agreement. Timely and scalable PCR tests are crucial for limiting further spread of monkeypox. The assay we provide streamlines high-throughput molecular testing for monkeypox virus on existing broadly established platforms used for SARS-CoV-2 diagnostic testing. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1080-6040 1080-6059 |
| Relation: | https://wwwnc.cdc.gov/eid/article/28/9/22-0917_article; https://doaj.org/toc/1080-6040; https://doaj.org/toc/1080-6059 |
| DOI: | 10.3201/eid2809.220917 |
| URL الوصول: | https://doaj.org/article/aca478786aa6465baf32eb3092b66b42 |
| رقم الأكسشن: | edsdoj.478786aa6465baf32eb3092b66b42 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10806040 10806059 |
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| DOI: | 10.3201/eid2809.220917 |