دورية أكاديمية
B Cell Subsets and Immune Checkpoint Expression in Patients with Chronic Lymphocytic Leukemia
| العنوان: | B Cell Subsets and Immune Checkpoint Expression in Patients with Chronic Lymphocytic Leukemia |
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| المؤلفون: | Aviwe Ntsethe, Zekhethelo Alondwe Mkhwanazi, Phiwayinkosi Vusi Dludla, Bongani Brian Nkambule |
| المصدر: | Current Issues in Molecular Biology, Vol 46, Iss 3, Pp 1731-1740 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | chronic lymphocytic leukemia, immune checkpoints, B cell subsets, beta-2 microglobulin, programmed death protein 1, cytotoxic T-lymphocyte-associated protein 4, Biology (General), QH301-705.5 |
| الوصف: | Chronic lymphocytic leukemia (CLL) is characterized by dysfunctional B cells. Immune checkpoint molecules such as cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed death-1 (PD-1) are upregulated in patients with CLL and may correlate with prognostic markers such as beta-2 microglobulin (B2M). The aim of this study was to evaluate the levels of immune checkpoints on B cell subsets and to further correlate them with B2M levels in patients with CLL. We recruited 21 patients with CLL and 12 controls. B cell subsets and the levels of immune checkpoint expression were determined using conventional multi-color flow cytometry. Basal levels of B2M in patients with CLL were measured using an enzyme-linked immunosorbent assay. Patients with CLL had increased levels of activated B cells when compared to the control group, p < 0.001. The expression of PD-1 and CTLA-4 were increased on activated B cells and memory B cells, p < 0.05. There were no associations between B2M levels and the measured immune checkpoints on B cell subsets, after adjusting for sex and age. In our cohort, the patients with CLL expressed elevated levels of PD-1 and CTLA-4 immune checkpoints on activated and memory B cell subsets. However, there was no correlation between these immune checkpoint expressions and B2M levels. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/46/3/112; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb46030112 |
| URL الوصول: | https://doaj.org/article/d4799eda6e56452d97842da2362a3e5e |
| رقم الأكسشن: | edsdoj.4799eda6e56452d97842da2362a3e5e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14673045 14673037 |
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| DOI: | 10.3390/cimb46030112 |