دورية أكاديمية
Genotype profiles of from gastric biopsies and strains with antimicrobial-induced resistance
| العنوان: | Genotype profiles of from gastric biopsies and strains with antimicrobial-induced resistance |
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| المؤلفون: | You-hua Wang, Fang-fei Wang, Xiao-ling Gong, Li-li Yan, Qiao-yun Zhao, Yan-ping Song, Ru-lin Zhao, Ya-jing He, Linfu Zhou, Dong-sheng Liu, Yong Xie |
| المصدر: | Therapeutic Advances in Gastroenterology, Vol 13 (2020) |
| بيانات النشر: | SAGE Publishing, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Diseases of the digestive system. Gastroenterology |
| مصطلحات موضوعية: | Diseases of the digestive system. Gastroenterology, RC799-869 |
| الوصف: | Background and aims: The genotypic method could significantly shorten the time needed to obtain antibiotic susceptibility data for Helicobacter pylori . The aim of this study was to explore the profile of H. pylori from gastric biopsies and strains with antibiotic-induced resistance. Methods: A total of 124 gastric biopsies were used to perform gene sequencing and to perform bacterial culture and susceptibility testing. Seven susceptible strains were selected to develop resistance to clarithromycin, levofloxacin, and metronidazole. Four susceptible strains were selected to transfer candidate mutations. The genotype profiles of these groups were analyzed by sequencing analysis. The antibiotic susceptibility of these strains was detected using the E-test method. Results: Phenotypic resistance to clarithromycin, levofloxacin, and metronidazole was observed in 35.5%, 40.0%, and 79.8% strains, respectively. Point mutations in 23 S rRNA, gyrA, and rdxA genes were observed in 39.5%, 38.7%, and 86.3% of gastric biopsies, respectively. The A2143G mutation in the 23S rRNA occurs in most clarithromycin-resistant samples. The A2142C point mutation showed a higher efficacy than A2142G and A2143G for inducing clarithromycin resistance. The D91N and N87K mutations in gyrA occurs in most levofloxacin-resistant samples, and double point mutations showed a higher efficacy than single mutations for inducing levofloxacin resistance. Phenotypic resistance and mutations in rdxA lacked consistency. Conclusion: Genotype-based gastric biopsy analysis was reliable for determining clarithromycin and levofloxacin resistance. A2143G in 23S rRNA and N87K/D91N in the gyrA gene occurred in most resistant strains. Mutations in the rdxA gene were not good indicators of metronidazole resistance. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-2848 17562848 |
| Relation: | https://doaj.org/toc/1756-2848 |
| DOI: | 10.1177/1756284820952596 |
| URL الوصول: | https://doaj.org/article/47fd151f062e4b328e777a501bf3d475 |
| رقم الأكسشن: | edsdoj.47fd151f062e4b328e777a501bf3d475 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17562848 |
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| DOI: | 10.1177/1756284820952596 |