دورية أكاديمية
PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer
| العنوان: | PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer |
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| المؤلفون: | Hanpu Zhang, Chenyuan Li, Shichong Liao, Yi Tu, Shengrong Sun, Feng Yao, Zhiyu Li, Zhong Wang |
| المصدر: | Cancer Biology & Therapy, Vol 23, Iss 1, Pp 1-11 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | psmd12, kif15, mek, erk, hepatocellular carcinoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | The tumor recurrence and drug resistance of hepatocellular carcinoma (HCC) threatened patients a lot. The mechanism should be further explored. The information of expression status and survival were available in public databases. The Western blot and immunohistochemistry staining displayed the level of related proteins. CCK-8, colony-formation assays, transwell assay and wound healing assay were performed to illustrate the ability of tumor growth, invasion and migration. In vivo model was established to verify our cell experiments. In our study, we revealed that proteasome 26S subunit, non-ATPase 12 (PSMD12) was high expressed in HCC tissues and positive related to the survival. In vitro experiments suggested that PSMD12 knockdown attenuated tumor cell growth, invasion and migration. Moreover, PSMD12 interference blocked the activation of MEK-ERK pathway. The ERK inhibitor could alleviate the tumor-promoting effect in PSMD12-overexpression cells. In addition, kinesin family member 15 (KIF15) was also observed to be highly expressed in HCC and be harmful to the survival. The public database, the images of immunohistochemistry and the western blot illustrated that PSMD12 and KIF15 was positive correlated. KIF15 knockdown impaired tumor progression and tumor-promoting effect of PSMD12. The xenograft models supported the results of cell experiments. In conclusion, PSMD12 could activated MEK-ERK pathway via KIF15 upregulation, thereby promoting tumor progression. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1538-4047 1555-8576 15384047 |
| Relation: | https://doaj.org/toc/1538-4047; https://doaj.org/toc/1555-8576 |
| DOI: | 10.1080/15384047.2022.2125260 |
| URL الوصول: | https://doaj.org/article/4818dd66f2db46008a876e186a5932ab |
| رقم الأكسشن: | edsdoj.4818dd66f2db46008a876e186a5932ab |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 15384047 15558576 |
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| DOI: | 10.1080/15384047.2022.2125260 |