دورية أكاديمية
In silico analysis of the Val66Met mutation in BDNF protein: implications for psychological stress
| العنوان: | In silico analysis of the Val66Met mutation in BDNF protein: implications for psychological stress |
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| المؤلفون: | Muhammad Adnan Shan, Muhammad Umer Khan, Warda Ishtiaq, Raima Rehman, Samiullah Khan, Muhammad Arshad Javed, Qurban Ali |
| المصدر: | AMB Express, Vol 14, Iss 1, Pp 1-16 (2024) |
| بيانات النشر: | SpringerOpen, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | BNDF V66M, Stress, Mutation, Proteins stability, Stress-related disorders, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract The brain-derived neurotrophic factor (BDNF) involves stress regulation and psychiatric disorders. The Val66Met polymorphism in the BDNF gene has been linked to altered protein function and susceptibility to stress-related conditions. This in silico analysis aimed to predict and analyze the consequences of the Val66Met mutation in the BDNF gene of stressed individuals. Computational techniques, including ab initio, comparative, and I-TASSER modeling, were used to evaluate the functional and stability effects of the Val66Met mutation in BDNF. The accuracy and reliability of the models were validated. Sequence alignment and secondary structure analysis compared amino acid residues and structural components. The phylogenetic analysis assessed the conservation of the mutation site. Functional and stability prediction analyses provided mixed results, suggesting potential effects on protein function and stability. Structural models revealed the importance of BDNF in key biological processes. Sequence alignment analysis showed the conservation of amino acid residues across species. Secondary structure analysis indicated minor differences between the wild-type and mutant forms. Phylogenetic analysis supported the evolutionary conservation of the mutation site. This computational study suggests that the Val66Met mutation in BDNF may have implications for protein stability, structural conformation, and function. Further experimental validation is needed to confirm these findings and elucidate the precise effects of this mutation on stress-related disorders. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-024-01664-w |
| URL الوصول: | https://doaj.org/article/4822b27f81c540d9b67c719bb36bb543 |
| رقم الأكسشن: | edsdoj.4822b27f81c540d9b67c719bb36bb543 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-024-01664-w |