دورية أكاديمية
Comparison of the Proteome of Huh7 Cells Transfected with Hepatitis B Virus Subgenotype A1, with or without G1862T
| العنوان: | Comparison of the Proteome of Huh7 Cells Transfected with Hepatitis B Virus Subgenotype A1, with or without G1862T |
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| المؤلفون: | Kiyasha Padarath, Aurélie Deroubaix, Previn Naicker, Stoyan Stoychev, Anna Kramvis |
| المصدر: | Current Issues in Molecular Biology, Vol 46, Iss 7, Pp 7032-7047 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | G1862T, HBV, subgenotype A1, p38 MAPK, replication protein A (RPA), DNA primase (PRIM2), Biology (General), QH301-705.5 |
| الوصف: | HBeAg is a non-structural, secreted protein of hepatitis B virus (HBV). Its p25 precursor is post-translationally modified in the endoplasmic reticulum. The G1862T precore mutation leads to the accumulation of P25 in the endoplasmic reticulum and activation of unfolded protein response. Using mass spectrometry, comparative proteome profiling of Huh-7 cells transfected with wildtype (WT) or G1862T revealed significantly differentially expressed proteins resulting in 12 dysregulated pathways unique to WT-transfected cells and 7 shared between cells transfected with either WT or G1862T. Except for the p38 MAPK signalling pathway, WT showed a higher number of DEPs than G1862T-transfected cells in all remaining six shared pathways. Two signalling pathways: oxidative stress and cell cycle signalling were differentially expressed only in cells transfected with G1862T. Fifteen pathways were dysregulated in G1862T-transfected cells compared to WT. The 15 dysregulated pathways were involved in the following processes: MAPK signalling, DNA synthesis and methylation, and extracellular matrix organization. Moreover, proteins involved in DNA synthesis signalling (replication protein A (RPA) and DNA primase (PRIM2)) were significantly upregulated in G1862T compared to WT. This upregulation was confirmed by mRNA quantification of both genes and immunofluorescent confocal microscopy for RPA only. The dysregulation of the pathways involved in these processes may lead to immune evasion, persistence, and uncontrolled proliferation, which are hallmarks of cancer. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 46070419 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/46/7/419; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb46070419 |
| URL الوصول: | https://doaj.org/article/48be2fdf14d24f99940ec619e6a77e40 |
| رقم الأكسشن: | edsdoj.48be2fdf14d24f99940ec619e6a77e40 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 46070419 14673045 14673037 |
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| DOI: | 10.3390/cimb46070419 |