دورية أكاديمية
Association of Stearoyl-CoA Desaturase Gene Polymorphisms on Milk Fatty Acid Composition of Holstein Friesian Cows in Indonesia
| العنوان: | Association of Stearoyl-CoA Desaturase Gene Polymorphisms on Milk Fatty Acid Composition of Holstein Friesian Cows in Indonesia |
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| المؤلفون: | R. Azis, A. Anggraeni |
| المصدر: | Tropical Animal Science Journal, Vol 46, Iss 1 (2023) |
| بيانات النشر: | IPB University, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | fatty acid, genotype, Holstein Friesian, RT-PCR, SCD gene, Animal culture, SF1-1100 |
| الوصف: | Milk fatty acid composition determines milk quality and the health of consumers. Stearoyl-CoA desaturase (SCD) gene controls lipid metabolism in the mammary gland, which plays an essential role in catalyzing saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). This study aimed to analyze the genetic diversity of the SCD gene and its effect on the milk fatty acid quality of Holstein Friesian (HF). Genetic variants were identified on 226 HF cattle from various dairy breeding Stations in West Java, Central Java, and East Java in Indonesia. Blood samples were extracted and amplified using Real-Time Polymerase Chain Reaction (RT-PCR) technique based on TaqMan Probe to determine the genotype of the SCD gene at g.10153A>G locus. Milk samples were analyzed by Gas Chromatography and Mass Spectrometry (GCMS) on 48 lactating cows. The SCD g.10153A>G produced three genotypes, namely AA (0.112), AG (0.640), and GG (0.271); and two alleles, namely A (0.409) and G (0.591). Genotype polymorphism of the SCD g.10153A>G had a significant effect (pG SNP (AG genotype) could be one of the candidates as a genetic marker to assist selection in reducing SFA and increasing MUFA in the milk content of HF cows in Indonesia. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2615-787X 2615-790X |
| Relation: | http://journal.ipb.ac.id/index.php/tasj/article/view/41530; https://doaj.org/toc/2615-787X; https://doaj.org/toc/2615-790X |
| DOI: | 10.5398/tasj.2023.46.1.27 |
| URL الوصول: | https://doaj.org/article/4965f769f69c4bc5bebabae8f45a0351 |
| رقم الأكسشن: | edsdoj.4965f769f69c4bc5bebabae8f45a0351 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2615787X 2615790X |
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| DOI: | 10.5398/tasj.2023.46.1.27 |