دورية أكاديمية
Hamster organotypic kidney culture model of early-stage SARS-CoV-2 infection highlights a two-step renal susceptibility
| العنوان: | Hamster organotypic kidney culture model of early-stage SARS-CoV-2 infection highlights a two-step renal susceptibility |
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| المؤلفون: | Sophie R Shyfrin, Marion Ferren, Laure Perrin-Cocon, Maxime Espi, Xavier Charmetant, Manon Brailly, Didier Decimo, Mathieu Iampietro, Lola Canus, Branka Horvat, Vincent Lotteau, Pierre-Olivier Vidalain, Olivier Thaunat, Cyrille Mathieu |
| المصدر: | Journal of Tissue Engineering, Vol 13 (2022) |
| بيانات النشر: | SAGE Publishing, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biochemistry |
| مصطلحات موضوعية: | Biochemistry, QD415-436 |
| الوصف: | Kidney pathology is frequently reported in patients hospitalized with COVID-19, the pandemic disease caused by the Severe acute respiratory coronavirus 2 (SARS-CoV-2). However, due to a lack of suitable study models, the events occurring in the kidney during the earliest stages of infection remain unknown. We have developed hamster organotypic kidney cultures (OKCs) to study the early stages of direct renal infection. OKCs maintained key renal structures in their native three-dimensional arrangement. SARS-CoV-2 productively replicated in hamster OKCs, initially targeting endothelial cells and later disseminating into proximal tubules. We observed a delayed interferon response, markers of necroptosis and pyroptosis, and an early repression of pro-inflammatory cytokines transcription followed by a strong later upregulation. While it remains an open question whether an active replication of SARS-CoV-2 takes place in the kidneys of COVID-19 patients with AKI, our model provides new insights into the kinetics of SARS-CoV-2 kidney infection and can serve as a powerful tool for studying kidney infection by other pathogens and testing the renal toxicity of drugs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2041-7314 20417314 |
| Relation: | https://doaj.org/toc/2041-7314 |
| DOI: | 10.1177/20417314221122130 |
| URL الوصول: | https://doaj.org/article/a499c83839eb4ee6895326bd1ac1aa35 |
| رقم الأكسشن: | edsdoj.499c83839eb4ee6895326bd1ac1aa35 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20417314 |
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| DOI: | 10.1177/20417314221122130 |