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Transcriptome analysis reveals the contribution of thermal and the specific effects in cellular response to millimeter wave exposure.

التفاصيل البيبلوغرافية
العنوان: Transcriptome analysis reveals the contribution of thermal and the specific effects in cellular response to millimeter wave exposure.
المؤلفون: Denis Habauzit, Catherine Le Quément, Maxim Zhadobov, Catherine Martin, Marc Aubry, Ronan Sauleau, Yves Le Dréan
المصدر: PLoS ONE, Vol 9, Iss 10, p e109435 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Radiofrequency radiations constitute a new form of environmental pollution. Among them, millimeter waves (MMW) will be widely used in the near future for high speed communication systems. This study aimed therefore to evaluate the biocompatibility of MMW at 60 GHz. For this purpose, we used a whole gene expression approach to assess the effect of acute 60 GHz exposure on primary cultures of human keratinocytes. Controls were performed to dissociate the electromagnetic from the thermal effect of MMW. Microarray data were validated by RT-PCR, in order to ensure the reproducibility of the results. MMW exposure at 20 mW/cm2, corresponding to the maximum incident power density authorized for public use (local exposure averaged over 1 cm2), led to an increase of temperature and to a strong modification of keratinocyte gene expression (665 genes differentially expressed). Nevertheless, when temperature is artificially maintained constant, no modification in gene expression was observed after MMW exposure. However, a heat shock control did not mimic exactly the MMW effect, suggesting a slight but specific electromagnetic effect under hyperthermia conditions (34 genes differentially expressed). By RT-PCR, we analyzed the time course of the transcriptomic response and 7 genes have been validated as differentially expressed: ADAMTS6, NOG, IL7R, FADD, JUNB, SNAI2 and HIST1H1A. Our data evidenced a specific electromagnetic effect of MMW, which is associated to the cellular response to hyperthermia. This study raises the question of co-exposures associating radiofrequencies and other environmental sources of cellular stress.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC4193780?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0109435
URL الوصول: https://doaj.org/article/49deeb7e38da4a629405a4451f350d61
رقم الأكسشن: edsdoj.49deeb7e38da4a629405a4451f350d61
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0109435