دورية أكاديمية
Performance of a Shotgun Prediction Model for Colorectal Cancer When Using 16S rRNA Sequencing Data
| العنوان: | Performance of a Shotgun Prediction Model for Colorectal Cancer When Using 16S rRNA Sequencing Data |
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| المؤلفون: | Elies Ramon, Mireia Obón-Santacana, Olfat Khannous-Lleiffe, Ester Saus, Toni Gabaldón, Elisabet Guinó, David Bars-Cortina, Gemma Ibáñez-Sanz, Lorena Rodríguez-Alonso, Alfredo Mata, Ana García-Rodríguez, Victor Moreno |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 2, p 1181 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | colon cancer, gut microbiota, shotgun, 16S, metagenomics, predictive model, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Colorectal cancer (CRC), the third most common cancer globally, has shown links to disturbed gut microbiota. While significant efforts have been made to establish a microbial signature indicative of CRC using shotgun metagenomic sequencing, the challenge lies in validating this signature with 16S ribosomal RNA (16S) gene sequencing. The primary obstacle is reconciling the differing outputs of these two methodologies, which often lead to divergent statistical models and conclusions. In this study, we introduce an algorithm designed to bridge this gap by mapping shotgun-derived taxa to their 16S counterparts. This mapping enables us to assess the predictive performance of a shotgun-based microbiome signature using 16S data. Our results demonstrate a reduction in performance when applying the 16S-mapped taxa in the shotgun prediction model, though it retains statistical significance. This suggests that while an exact match between shotgun and 16S data may not yet be feasible, our approach provides a viable method for comparative analysis and validation in the context of CRC-associated microbiome research. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/2/1181; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25021181 |
| URL الوصول: | https://doaj.org/article/d49e5bc3e2644a86b5b2e66880c3cd22 |
| رقم الأكسشن: | edsdoj.49e5bc3e2644a86b5b2e66880c3cd22 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25021181 |