دورية أكاديمية
Next-Generation Sequencing Profiles of the Methylome and Transcriptome in Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis
| العنوان: | Next-Generation Sequencing Profiles of the Methylome and Transcriptome in Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis |
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| المؤلفون: | Chia-Chun Tseng, Yuan-Zhao Lin, Chia-Hui Lin, Ruei-Nian Li, Chang-Yi Yen, Hua-Chen Chan, Wen-Chan Tsai, Tsan-Teng Ou, Cheng-Chin Wu, Wan-Yu Sung, Jeng-Hsien Yen |
| المصدر: | Journal of Clinical Medicine, Vol 8, Iss 9, p 1284 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | rheumatoid arthritis, methylation, next-generation sequencing, Medicine |
| الوصف: | Using next-generation sequencing to decipher methylome and transcriptome and underlying molecular mechanisms contributing to rheumatoid arthritis (RA) for improving future therapies, we performed methyl-seq and RNA-seq on peripheral blood mononuclear cells (PBMCs) from RA subjects and normal donors. Principal component analysis and hierarchical clustering revealed distinct methylation signatures in RA with methylation aberrations noted across chromosomes. Methylation alterations varied with CpG features and genic characteristics. Typically, CpG islands and CpG shores were hypermethylated and displayed the greatest methylation variance. Promoters were hypermethylated and enhancers/gene bodies were hypomethylated, with methylation variance associated with expression variance. RA genetically associated genes preferentially displayed differential methylation and differential expression or interacted with differentially methylated and differentially expressed genes. These differentially methylated and differentially expressed genes were enriched with several signaling pathways and disease categories. 10 genes (CD86, RAB20, XAF1, FOLR3, LTBR, KCNH8, DOK7, PDGFA, PITPNM2, CELSR1) with concomitantly differential methylation in enhancers/promoters/gene bodies and differential expression in B cells were validated. This integrated analysis of methylome and transcriptome identified novel epigenetic signatures associated with RA and highlighted the interaction between genetics and epigenetics in RA. These findings help our understanding of the pathogenesis of RA and advance epigenetic studies in regards to the disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2077-0383 83838627 |
| Relation: | https://www.mdpi.com/2077-0383/8/9/1284; https://doaj.org/toc/2077-0383 |
| DOI: | 10.3390/jcm8091284 |
| URL الوصول: | https://doaj.org/article/cd4a89a23e6f4f83838627e8356c2f71 |
| رقم الأكسشن: | edsdoj.4a89a23e6f4f83838627e8356c2f71 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20770383 83838627 |
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| DOI: | 10.3390/jcm8091284 |