دورية أكاديمية
أعرض في EDS

Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease

التفاصيل البيبلوغرافية
العنوان: Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease
المؤلفون: Xin Wang, Huizhen Wang, Jiawen Li, Lanying Li, Yifan Wang, Aiqing Li
المصدر: Renal Failure, Vol 45, Iss 1 (2023)
بيانات النشر: Taylor & Francis Group, 2023.
سنة النشر: 2023
المجموعة: LCC:Diseases of the genitourinary system. Urology
مصطلحات موضوعية: High-salt diet, chronic kidney disease, subfornical organ, phosphopeptides, Diseases of the genitourinary system. Urology, RC870-923
الوصف: AbstractObjectives Subfornical organ (SFO) is vital in chronic kidney disease (CKD) progression caused by high salt levels. The current study investigated the effects of high salt on phosphoproteomic changes in SFO in CKD rats.Methods 5/6 nephrectomized rats were fed a normal-salt diet (0.4%) (NC group) or a high-salt diet (4%) (HC group) for three weeks, while sham-operated rats were fed a normal-salt diet (0.4%) (NS group). For phosphoproteomic analysis of SFO in different groups, TiO2 enrichment, isobaric tags for relative and absolute quantification (iTRAQ) labeling, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used.Results There were 6808 distinct phosphopeptides found, which corresponded to 2661 phosphoproteins. NC group had 168 upregulated and 250 downregulated phosphopeptides compared to NS group. Comparison to NC group, HC group had 154 upregulated and 124 downregulated phosphopeptides. Growth associated protein 43 (GAP43) and heat shock protein 27 (Hsp27) were significantly upregulated phosphoproteins and may protect against high-salt damage. Differential phosphoproteins with tight functional connection were synapse proteins and microtubule-associated proteins, implying that high-salt diet disrupted brain’s structure and function. Furthermore, differential phosphoproteins in HC/NC comparison group were annotated to participate in GABAergic synapse signaling pathway and aldosterone synthesis and secretion, which attenuated inhibitory neurotransmitter effects and increased sympathetic nerve activity (SNA).Discussion This large scale phosphoproteomic profiling of SFO sheds light on how salt aggravates CKD via the central nervous system.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0886022X
1525-6049
0886-022X
Relation: https://doaj.org/toc/0886-022X; https://doaj.org/toc/1525-6049
DOI: 10.1080/0886022X.2023.2171886
URL الوصول: https://doaj.org/article/4a98659ca6164c96b3dd7542d7c12516
رقم الأكسشن: edsdoj.4a98659ca6164c96b3dd7542d7c12516
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:0886022X
15256049
DOI:10.1080/0886022X.2023.2171886