دورية أكاديمية
miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer
| العنوان: | miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer |
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| المؤلفون: | Xiangyu Zhu, Si-ping Ma, Dongxiang Yang, Yanlong Liu, Yong-peng Wang, Tao Lin, Yan-xi Li, Shi-hua Yang, Wan-chuan Zhang, Xin-ling Wang |
| المصدر: | Cellular Physiology and Biochemistry, Vol 51, Iss 4, Pp 1969-1981 (2018) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Physiology LCC:Biochemistry |
| مصطلحات موضوعية: | Colorectal cancer, miR-142-3p, CDK4, Cell cycle, Physiology, QP1-981, Biochemistry, QD415-436 |
| الوصف: | Background/Aims: Deregulation of microRNAs (miRNAs) has been associated with a variety of cancers, including colorectal cancer (CRC). Here, we investigated anomalous miR-142-3p expression and its possible functional consequences in primary CRC samples. Methods: The expression of miR-142-3p was measured by quantitative RT-PCR in 116 primary CRC tissues and adjacent non-tumor tissues. The effect of miR-142-3p up- or down-regulation in CRC-derived cells was evaluated in vitro by cell viability and colony formation assays and in vivo by growth assays in xenografted nude mice. Results: Using quantitative RT-PCR, we found that miR-142-3p was down-regulated in 78.4 % (91/116) of the primary CRC tissues tested when compared to the adjacent non-tumor tissues. We also found that the miR-142-3p mimic reduced in vitro cell viability and colony formation by inducing cell cycle arrest in CRC-derived cells, and inhibited in vivo tumor cell growth in xenografted nude mice. Inversely, we found that the miR-142-3p inhibitor increased the viability and colony forming capacity of CRC-derived cells and tumor cell growth in xenografted nude mice. In addition, we identified CDK4 as a potential target of miR-142-3p by predictions and dual-luciferase reporter assays. Concordantly, we found that miR-142-3p mimics and inhibitors could decrease and increase CDK4 protein levels in CRC-derived cells, respectively. Conclusion: From our results we conclude that miR-142-3p may act as a tumor suppressor in CRC and may serve as a tool for miRNA-based CRC therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1015-8987 1421-9778 |
| Relation: | https://www.karger.com/Article/FullText/495721; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000495721 |
| URL الوصول: | https://doaj.org/article/4b151e0b41d248a8b532c9a85d9b3735 |
| رقم الأكسشن: | edsdoj.4b151e0b41d248a8b532c9a85d9b3735 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10158987 14219778 |
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| DOI: | 10.1159/000495721 |