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Whole genome sequencing unravels cryptic circulation of divergent dengue virus lineages in the rainforest region of Nigeria

التفاصيل البيبلوغرافية
العنوان: Whole genome sequencing unravels cryptic circulation of divergent dengue virus lineages in the rainforest region of Nigeria
المؤلفون: Bernard Anyebe Onoja, Judith Uche Oguzie, Uwem Etop George, Kaego Emmanuel Asoh, Philip Ajayi, Toluwanimi Faithful Omofaye, Imafidon Oluwatoyin Igeleke, Philomena Eromon, Soumare Harouna, Edyth Parker, Adekunle Johnson Adeniji, Christian T. Happi
المصدر: Emerging Microbes and Infections, Vol 13, Iss 1 (2024)
بيانات النشر: Taylor & Francis Group, 2024.
سنة النشر: 2024
المجموعة: LCC:Infectious and parasitic diseases
LCC:Microbiology
مصطلحات موضوعية: Dengue, IgG, IgM, NS1, genomic sequencing, NGS, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
الوصف: ABSTRACTDengue is often misclassified and underreported in Africa due to inaccurate differential diagnoses of nonspecific febrile illnesses such as malaria, sparsity of diagnostic testing and poor clinical and genomic surveillance. There are limited reports on the seroprevalence and genetic diversity of dengue virus (DENV) in humans and vectors in Nigeria. In this study, we investigated the epidemiology and genetic diversity of dengue in the rainforest region of Nigeria. We screened 515 febrile patients who tested negative for malaria and typhoid fever in three hospitals in Oyo and Ekiti States in southern Nigeria with a combination of anti-dengue IgG/IgM/NS1 rapid test kits and metagenomic sequencing. We found that approximately 28% of screened patients had previous DENV exposure, with the highest prevalence in persons over sixty. Approximately 8% of the patients showed evidence of recent or current infection, and 2.7% had acute infection. Following sequencing of sixty samples, we assembled twenty DENV-1 genomes (3 complete and 17 partial). We found that all assembled genomes belonged to DENV-1 genotype III. Our phylogenetic analyses showed evidence of prolonged cryptic circulation of divergent DENV lineages in Oyo state. We were unable to resolve the source of DENV in Nigeria owing to limited sequencing data from the region. However, our sequences clustered closely with sequences in Tanzania and sequences reported in Chinese with travel history to Tanzania in 2019. This may reflect the wider unsampled bidirectional transmission of DENV-1 in Africa, which strongly emphasizes the importance of genomic surveillance in monitoring ongoing DENV transmission in Africa.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 22221751
2222-1751
Relation: https://doaj.org/toc/2222-1751
DOI: 10.1080/22221751.2024.2307511
URL الوصول: https://doaj.org/article/4b46803bd9894bc98557ddc12c15da99
رقم الأكسشن: edsdoj.4b46803bd9894bc98557ddc12c15da99
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22221751
DOI:10.1080/22221751.2024.2307511