دورية أكاديمية
The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation
العنوان: | The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation |
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المؤلفون: | Ingrid Rössler, Sarah Weigl, José Fernández-Fernández, Sara Martín-Villanueva, Daniela Strauss, Ed Hurt, Jesús de la Cruz, Brigitte Pertschy |
المصدر: | RNA Biology, Vol 19, Iss 1, Pp 560-574 (2022) |
بيانات النشر: | Taylor & Francis Group, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Genetics |
مصطلحات موضوعية: | ribosome biogenesis, 40s ribosomal subunit, ribosomal protein, rps15/us19, yeast, chronic lymphocytic leukaemia, cll, Genetics, QH426-470 |
الوصف: | The small ribosomal subunit protein Rps15/uS19 is involved in early nucleolar ribosome biogenesis and subsequent nuclear export of pre-40S particles to the cytoplasm. In addition, the C-terminal tail of Rps15 was suggested to play a role in mature ribosomes, namely during translation elongation. Here, we show that Rps15 not only functions in nucleolar ribosome assembly but also in cytoplasmic pre-40S maturation, which is indicated by a strong genetic interaction between Rps15 and the 40S assembly factor Ltv1. Specifically, mutations either in the globular or C-terminal domain of Rps15 when combined with the non-essential ltv1 null allele are lethal or display a strong growth defect. However, not only rps15 ltv1 double mutants but also single rps15 C-terminal deletion mutants exhibit an accumulation of the 20S pre-rRNA in the cytoplasm, indicative of a cytoplasmic pre-40S maturation defect. Since in pre-40S particles, the C-terminal tail of Rps15 is positioned between assembly factors Rio2 and Tsr1, we further tested whether Tsr1 is genetically linked to Rps15, which indeed could be demonstrated. Thus, the integrity of the Rps15 C-terminal tail plays an important role during late pre-40S maturation, perhaps in a quality control step to ensure that only 40S ribosomal subunits with functional Rps15 C-terminal tail can efficiently enter translation. As mutations in the C-terminal tail of human RPS15 have been observed in connection with chronic lymphocytic leukaemia, it is possible that apart from defects in translation, an impaired late pre-40S maturation step in the cytoplasm could also be a reason for this disease. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1547-6286 1555-8584 15476286 |
Relation: | https://doaj.org/toc/1547-6286; https://doaj.org/toc/1555-8584 |
DOI: | 10.1080/15476286.2022.2064073 |
URL الوصول: | https://doaj.org/article/d4beb46d371a4252ad0cc5a7a6b25026 |
رقم الأكسشن: | edsdoj.4beb46d371a4252ad0cc5a7a6b25026 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15476286 15558584 |
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DOI: | 10.1080/15476286.2022.2064073 |