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Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection

التفاصيل البيبلوغرافية
العنوان: Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection
المؤلفون: Xuehua Zhan, Wenqi Yuan, Yueyong Zhou, Rong Ma, Zhaohui Ge
المصدر: BMC Genomics, Vol 23, Iss 1, Pp 1-17 (2022)
بيانات النشر: BMC, 2022.
سنة النشر: 2022
المجموعة: LCC:Biotechnology
LCC:Genetics
مصطلحات موضوعية: Exosomal miRNA, Tuberculosis, BCG, Macrophages, Apoptosis, RNA-seq, Biotechnology, TP248.13-248.65, Genetics, QH426-470
الوصف: Abstract Background The mechanisms through which Mycobacterium tuberculosis evades immune surveillance during tuberculosis (TB) infection remain complex. Previous studies have found that Mycobacteria can manipulate the miRNAs of host cells to promote their survival during host-pathogen interactions, and most of these effects occur at the cellular miRNA level. We attempted to investigate the possible related mechanisms at the exosomal miRNA level. Results High-throughput sequencing revealed that Bacillus Calmette-Guérin (BCG) infection could alter the composition of the macrophage exosome content, and the expression levels of miRNAs in exosomes derived from the cell culture media of macrophages showed significant differences between the BCG-infected and non-infected groups. Compared with the non-infected group, 20 exosomal miRNAs were up-regulated and 7 exosomal miRNAs were down-regulated in the infection group (p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1471-2164
Relation: https://doaj.org/toc/1471-2164
DOI: 10.1186/s12864-022-08590-w
URL الوصول: https://doaj.org/article/e4d5ebe6d0694c5bb1c422bfd9e50010
رقم الأكسشن: edsdoj.4d5ebe6d0694c5bb1c422bfd9e50010
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14712164
DOI:10.1186/s12864-022-08590-w