دورية أكاديمية
PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis
| العنوان: | PARP-1, EpCAM, and FRα as potential targets for intraoperative detection and delineation of endometriosis: a quantitative tissue expression analysis |
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| المؤلفون: | Beatrice Belmonte, Giovanni Di Lorenzo, Alessandro Mangogna, Barbara Bortot, Giorgio Bertolazzi, Selene Sammataro, Simona Merighi, Anna Martorana, Gabriella Zito, Federico Romano, Anna Giorgiutti, Cristina Bottin, Fabrizio Zanconati, Andrea Romano, Giuseppe Ricci, Stefania Biffi |
| المصدر: | Reproductive Biology and Endocrinology, Vol 22, Iss 1, Pp 1-12 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Gynecology and obstetrics LCC:Reproduction |
| مصطلحات موضوعية: | PARP-1, EpCAM, FRα, Endometriosis, Intraoperative imaging, Tissue expression, Gynecology and obstetrics, RG1-991, Reproduction, QH471-489 |
| الوصف: | Abstract Background Endometriosis is a gynecological disease characterized by the presence of endometrial tissue in abnormal locations, leading to severe symptoms, inflammation, pain, organ dysfunction, and infertility. Surgical removal of endometriosis lesions is crucial for improving pain and fertility outcomes, with the goal of complete lesion removal. This study aimed to analyze the location and expression patterns of poly (ADP-ribose) polymerase 1 (PARP-1), epithelial cell adhesion molecule (EpCAM), and folate receptor alpha (FRα) in endometriosis lesions and evaluate their potential for targeted imaging. Methods Gene expression analysis was performed using the Turku endometriosis database (EndometDB). By immunohistochemistry, we investigated the presence and distribution of PARP-1, EpCAM, and FRα in endometriosis foci and adjacent tissue. We also applied an ad hoc platform for the analysis of images to perform a quantitative immunolocalization analysis. Double immunofluorescence analysis was carried out for PARP-1 and EpCAM, as well as for PARP-1 and FRα, to explore the expression of these combined markers within endometriosis foci and their potential simultaneous utilization in surgical treatment. Results Gene expression analysis revealed that PARP-1, EpCAM, and FOLR1 (FRα gene) are more highly expressed in endometriotic lesions than in the peritoneum, which served as the control tissue. The results of the immunohistochemical study revealed a significant increase in the expression levels of all three biomarkers inside the endometriosis foci compared to the adjacent tissues. Additionally, the double immunofluorescence analysis consistently demonstrated the presence of PARP-1 in the nucleus and the expression of EpCAM and FRα in the cell membrane and cytoplasm. Conclusion Overall, these three markers demonstrate significant potential for effective imaging of endometriosis. In particular, the results emphasize the importance of PARP-1 expression as a possible indicator for distinguishing endometriotic lesions from adjacent tissue. PARP-1, as a potential biomarker for endometriosis, offers promising avenues for further investigation in terms of both pathophysiology and diagnostic-therapeutic approaches. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-7827 |
| Relation: | https://doaj.org/toc/1477-7827 |
| DOI: | 10.1186/s12958-024-01264-0 |
| URL الوصول: | https://doaj.org/article/4d6749387fc843eb9f92e95d8cc5daad |
| رقم الأكسشن: | edsdoj.4d6749387fc843eb9f92e95d8cc5daad |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14777827 |
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| DOI: | 10.1186/s12958-024-01264-0 |