دورية أكاديمية
أعرض في EDS

Genome-Wide Analysis of DNA Methylation in Human Amnion

التفاصيل البيبلوغرافية
العنوان: Genome-Wide Analysis of DNA Methylation in Human Amnion
المؤلفون: Jinsil Kim, Mitchell M. Pitlick, Paul J. Christine, Amanda R. Schaefer, Cesar Saleme, Belén Comas, Viviana Cosentino, Enrique Gadow, Jeffrey C. Murray
المصدر: The Scientific World Journal, Vol 2013 (2013)
بيانات النشر: Wiley, 2013.
سنة النشر: 2013
المجموعة: LCC:Technology
LCC:Medicine
LCC:Science
مصطلحات موضوعية: Technology, Medicine, Science
الوصف: The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1537-744X
Relation: https://doaj.org/toc/1537-744X
DOI: 10.1155/2013/678156
URL الوصول: https://doaj.org/article/4da63e779a994d909ce8a773c9d85f07
رقم الأكسشن: edsdoj.4da63e779a994d909ce8a773c9d85f07
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1537744X
DOI:10.1155/2013/678156