The study was designed to examine the effect of sodium bentonite (SB) as mycotoxin binder in poultry feed and its effects on productions performance of laying hens. A total of forty-five production hens white leghorn aged 34 weeks were caged in a naturally aired laying house into five different groups. Group A was kept as control group, Group B was fed with higher level 170 ppb aflatoxin without binders, and Group C feed contained 170 ppb aflatoxin with 1.5% SB binder, Group D contained 170 ppb aflatoxin with 2% SB and Group E feed contained 170 ppb aflatoxin with 2.5% SB. Aflatoxin contamination adversely affected the egg production and feed consumption. Addition of 2% and 2.5% SB increased (P