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NotI Microarrays: Novel Epigenetic Markers for Early Detection and Prognosis of High Grade Serous Ovarian Cancer

التفاصيل البيبلوغرافية
العنوان: NotI Microarrays: Novel Epigenetic Markers for Early Detection and Prognosis of High Grade Serous Ovarian Cancer
المؤلفون: Vera N. Senchenko, Eugene Zabarovsky, Michael Lerman, Surya P. Yenamandra, Eleonora A. Braga, Anna V. Gerashchenko, Vasily V. Gordiyuk, Ilya Ignatjev, George S. Krasnov, Tatiana Pavlova, Vladimir Kashuba, Alexey A. Dmitriev
المصدر: International Journal of Molecular Sciences, Vol 13, Iss 10, Pp 13352-13377 (2012)
بيانات النشر: MDPI AG, 2012.
سنة النشر: 2012
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: ovarian cancer, biomarkers, NotI microarrays, epigenetics, early detection of ovarian cancer, prognosis of ovarian cancer, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Chromosome 3-specific NotI microarray (NMA) containing 180 clones with 188 genes was used in the study to analyze 18 high grade serous ovarian cancer (HGSOC) samples and 7 benign ovarian tumors. We aimed to find novel methylation-dependent biomarkers for early detection and prognosis of HGSOC. Thirty five NotI markers showed frequency of methylation/deletion more or equal to 17%. To check the results of NMA hybridizations several samples for four genes (LRRC3B, THRB, ITGA9 and RBSP3 (CTDSPL)) were bisulfite sequenced and confirmed the results of NMA hybridization. A set of eight biomarkers: NKIRAS1/RPL15, THRB, RBPS3 (CTDSPL), IQSEC1, NBEAL2, ZIC4, LOC285205 and FOXP1, was identified as the most prominent set capable to detect both early and late stages of ovarian cancer. Sensitivity of this set is equal to (72 ± 11)% and specificity (94 ± 5)%. Early stages represented the most complicated cases for detection. To distinguish between Stages I + II and Stages III + IV of ovarian cancer the most perspective set of biomarkers would include LOC285205, CGGBP1, EPHB1 and NKIRAS1/RPL15. The sensitivity of the set is equal to (80 ± 13)% and the specificity is (88 ± 12)%. Using this technique we plan to validate this panel with new epithelial ovarian cancer samples and add markers from other chromosomes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/13/10/13352; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms131013352
URL الوصول: https://doaj.org/article/4e00220ee5524414b899283209656a74
رقم الأكسشن: edsdoj.4e00220ee5524414b899283209656a74
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms131013352