دورية أكاديمية
Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays
| العنوان: | Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays |
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| المؤلفون: | Carlos Alvarez-Moya, Mónica Reynoso Silva, Alma Rosa Villalobos Arámbula, Alfonso Islas Sandoval, Hugo Castañeda Vasquez, Rosa María González Montes |
| المصدر: | Genetics and Molecular Biology, Vol 34, Iss 1, Pp 127-130 (2011) |
| بيانات النشر: | Sociedade Brasileira de Genética, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | glyphosate, DNA damage, comet assay, plant genotoxicity test, Tradescantia point mutation test, Genetics, QH426-470 |
| الوصف: | Glyphosate is noted for being non-toxic in fishes, birds and mammals (including humans). Nevertheless, the degree of genotoxicity is seriously controversial. In this work, various concentrations of a glyphosate isopropylamine salt were tested using two methods of genotoxicity assaying, viz., the pink mutation assay with Tradescantia (4430) and the comet assay with nuclei from staminal cells of the same plant. Staminal nuclei were studied in two different forms, namely nuclei from exposed plants, and nuclei exposed directly. Using the pink mutation assay, isopropylamine induced a total or partial loss of color in staminal cells, a fundamental criterion utilized in this test. Consequently, its use is not recommended when studying genotoxicity with agents that produce pallid staminal cells. The comet assay system detected statistically significant (p < 0.01) genotoxic activity by isopropylamine, when compared to the negative control in both the nuclei of treated plants and directly treated nuclei, but only the treated nuclei showed a dose-dependent increase. Average migration in the nuclei of treated plants increased, when compared to that in treated nuclei. This was probably due, either to the permanence of isopropylamine in inflorescences, or to the presence of secondary metabolites. In conclusion, isopropylamine possesses strong genotoxic activity, but its detection can vary depending on the test systems used. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1415-4757 1678-4685 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100022; https://doaj.org/toc/1415-4757; https://doaj.org/toc/1678-4685 |
| URL الوصول: | https://doaj.org/article/4e3c8b051a184a9d82c854cea937a489 |
| رقم الأكسشن: | edsdoj.4e3c8b051a184a9d82c854cea937a489 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14154757 16784685 |
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