دورية أكاديمية
Optimization of Enzyme Assisted Alkaline Extraction of Sunflower (helianthus Annuus L.) Protein for Alternative Isolate Production
| العنوان: | Optimization of Enzyme Assisted Alkaline Extraction of Sunflower (helianthus Annuus L.) Protein for Alternative Isolate Production |
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| المؤلفون: | Dmitry V. Baurin, Alexandra V. Baurina, Irina V. Shakir, Victor I. Panfilov |
| المصدر: | Chemical Engineering Transactions, Vol 93 (2022) |
| بيانات النشر: | AIDIC Servizi S.r.l., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Chemical engineering LCC:Computer engineering. Computer hardware |
| مصطلحات موضوعية: | Chemical engineering, TP155-156, Computer engineering. Computer hardware, TK7885-7895 |
| الوصف: | This research focused on the effect of extraction time (60–120 min), enzyme/substrate ratio (0.05–0.4 %) temperature (37.5°C) on enzyme assisted alkaline protein extraction efficiency from non-dehulled sunflower meal, which is a by-product of oil production. 4 commercial enzymes were studied and the highest protein yield (92 %) was obtained by ultrafiltration of the enzymatic hydrolysates through a membrane (selectivity 81.4%) followed by the isoelectric precipitation (pH 4.5) and vacuum drying. The protein yield was found 92.85% at the optimized condition of enzyme assisted alkaline extraction: temperature 37.5 °C, 60 min, and enzyme/substrate ratio of 0.2%. Compared to alkaline extraction with 0.25% NaOH and no enzyme addition, the protein yield has increased by 31% due to the mild extraction conditions. Determined physicochemical properties of the obtained protein isolate: high protein content (92.85% d.b) as well as high solubility (95.0% at pH 7.5) reflect the unique characteristics of the product. However, the process requires further optimization because of the green colour of the isolate, which limits its application in the food industry. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2283-9216 |
| Relation: | https://www.cetjournal.it/index.php/cet/article/view/12527; https://doaj.org/toc/2283-9216 |
| DOI: | 10.3303/CET2293030 |
| URL الوصول: | https://doaj.org/article/4e4c55bccf134889841adc78b3a8363d |
| رقم الأكسشن: | edsdoj.4e4c55bccf134889841adc78b3a8363d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22839216 |
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| DOI: | 10.3303/CET2293030 |